Recombinant Full Length Human PPM1D Protein, C-Flag-tagged

• Cat.No.: PPM1D-324HFL
• Product Overview: Recombinant Full Length Human PPM1D Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 66.5 kDa
• AA Sequence: MAGLYSLGVSVFSDQGGRKYMEDVT QIVVEPEPTAEEKPSPRRSLSQPLP PRPSPAALPGGEVSGKGPAV AAREARDPLPDAGASPAPSRCCRRR SSVAFFAVCDGHGGREAAQFAREHL WGFIKKQKGFTSSEPAKVCA AIRKGFLACHLAMWKKLAEWPKTMT GLPSTSGTTASVVIIRGMKMYVAHV GDSGVVLGIQDDPKDDFVRA VEVTQDHKPELPKERERIEGLGGSV MNKSGVNRVVWKRPRLTHNGPVRRS TVIDQIPFLAVARALGDLWS YDFFSGEFVVSPEPDTSVHTLDPQK HKYIILGSDGLWNMIPPQDAISMCQ DQEEKKYLMGEHGQSCAKML VNRALGRWRQRMLRADNTSAIVICI SPEVDNQGNFTNEDELYLNLTDSPS YNSQETCVMTPSPCSTPPVK SLEEDPWPRVNSKDHIPALVRSNAF SENFLEVSAEIARENVQGVVIPSKD PEPLEENCAKALTLRIHDSL NNSLPIGLVPTNSTNTVMDQKNLKM STPGQMKAQEIERTPPTNFKRTLEE SNSGPLMKKHRRNGLSRSSG
  AQPASLPTTSQRKNSVKLTMRRRLR GQKKIGNPLLHQHRKTVCVCTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: p53 signaling pathway

Gene Information

• Gene Name: PPM1D protein phosphatase, Mg2+/Mn2+ dependent 1D [ Homo sapiens (human) ]
• Official Symbol: PPM1D
• Synonyms: JDVS; WIP1; IDDGIP; PP2C-DELTA
• Gene ID: 8493
• mRNA Refseq: NM_003620.4
• Protein Refseq: NP_003611.1
• MIM: 605100
• UniProt ID: O15297