Recombinant Full Length Human SMAD3 Protein, GST-tagged SMAD3-1973HF

Recombinant Full Length Human SMAD3 Protein, GST-tagged

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Cat.No. : SMAD3-1973HF Optional Service: Optional requirements on this protein
Product Overview : Full-length human Smad3 [1-425(end) amino acids of accession number NP_005893.1] was expressed as N-terminal GST-fusion protein (75 kDa) using E.coli expression system. GST-Smad3 was purified by using glutathione sepharose chromatography
Source : E. coli
Species : Human
Tag : GST
Form : 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Bio-activity : The phosphorylational ability of the protein was determined by ELISA. The substrate protein was incubated with the indicated enzyme, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
Protein length : 1-425(end)
Purity : 61%
Applications : SDS-PAGE
Storage : Store at -80C. Avoid repeating freeze-thaws.
SDS-PAGE :

The purity was assessed by SDS-PAGE/CBB staining.

Activity data :

The phosphorylational ability of the protein was determined by ELISA. The substrate protein was incubated with the indicated enzyme, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).

Gene Name : SMAD family member 3
Official Symbol : SMAD3
Synonyms : MADH3; JV15-2; HsT17436
Protein Refseq : NP_005893.1
UniProt ID : Q9GKR4
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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