| Cat.No. : |
SMAD3-1973HF
Optional Service: Optional requirements on this protein
|
| Product Overview : |
Full-length human Smad3 [1-425(end) amino acids of accession number NP_005893.1] was expressed as N-terminal GST-fusion protein (75 kDa) using E.coli expression system. GST-Smad3 was purified by using glutathione sepharose chromatography |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST |
| Form : |
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5 |
| Bio-activity : |
The phosphorylational ability of the protein was determined by ELISA. The substrate protein was incubated with the indicated enzyme, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). |
| Protein length : |
1-425(end) |
| Purity : |
61% |
| Applications : |
SDS-PAGE |
| Storage : |
Store at -80C. Avoid repeating freeze-thaws. |
| SDS-PAGE : |
The purity was assessed by SDS-PAGE/CBB staining.
|
| Activity data : |
The phosphorylational ability of the protein was determined by ELISA. The substrate protein was incubated with the indicated enzyme, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
