Active Recombinant Human ACE Protein, His-tagged

• Cat.No.: ACE-033H
• Product Overview: Recombinant human ACE/CD143, fused to His-tag at Cterminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes an enzyme involved in blood pressure regulation and electrolyte balance. It catalyzes the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This angiotensin converting enzyme (ACE) also inactivates the vasodilator protein, bradykinin. Accordingly, the encoded enzyme increases blood pressure and is a drug target of ACE inhibitors, which are often prescribed to reduce blood pressure. This enzyme additionally plays a role in fertility through its ability to cleave and release GPI-anchored membrane proteins in spermatozoa. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme. This polymorphism, as well as mutations in this gene, have been implicated in a wide variety of diseases including cardiovascular pathophysiologies, psoriasis, renal disease, stroke, and Alzheimer's disease. Regulation of the homologous ACE2 gene may be involved in progression of disease caused by several human coronaviruses, including SARS-CoV and SARS-CoV-2. Alternative splicing results in multiple transcript variants encoding both somatic (sACE) and male-specific testicular (tACE) isoforms.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is > 1,000 pmol/min/mg, and is defined as the amount of enzyme that cleaves 1 pmol of Mca-RPPGFSAFK(Dnp)-OH per minute at 25 centigrade.
• Molecular Mass: 142kDa
• AA Sequence: LDPGLQPGNFSADEAGAQLFAQSYN SSAEQVLFQSVAASWAHDTNITAEN ARRQEEAALLSQEFAEAWGQKAKEL YEPIWQNFTDPQLRRIIGAVRTLGS ANLPLAKRQQYNALLSNMSRIYSTA KVCLPNKTATCWSLDPDLTNILASS RSYAMLLFAWEGWHNAAGIPLKPLY EDFTALSNEAYKQDGFTDTGAYWRS WYNSPTFEDDLEHLYQQLEPLYLNL HAFVRRALHRRYGDRYINLRGPIPA HLLGDMWAQSWENIYDMVVPFPDKP NLDVTSTMLQQGWNATHMFRVAEEF FTSLELSPMPPEFWEGSMLEKPADG REVVCHASAWDFYNRKDFRIKQCTR VTMDQLSTVHHEMGHIQYYLQYKDL PVSLRRGANPGFHEAIGDVLALSVS TPEHLHKIGLLDRVTNDTESDINYL LKMALEKIAFLPFGYLVDQWRWGVF SGRTPPSRYNFDWWYLRTKYQGICP PVTRNETHFDAGAKFHVPNVTPYIR YFVSFVLQFQFHEALCKEAGYEGPL HQCDIYRSTKAGAKLRKVLQAGSSR PWQEVLKDMVGLDALDAQPLLKYFQ PVTQWLQEQNQQNGEVLGWPEYQWH PPLPDNYPEGIDLVTDEAEASKFVE EYDRTSQVVWNEYAEANWNYNTNIT TETSKILLQKNMQIANHTLKYGTQA RKFDVNQLQNTTIKRIIKKVQDLER AALPAQELEEYNKILLDMETTYSVA TVCHPNGSCLQLEPDLTNVMATSRK YEDLLWAWEGWRDKAGRAILQFYPK YVELINQAARLNGYVDAGDSWRSMY ETPSLEQDLERLFQELQPLYLNLHA YVRRALHRHYGAQHINLEGPIPAHL LGNMWAQTWSNIYDLVVPFPSAPSM DTTEAMLKQGWTPRRMFKEADDFFT SLGLLPVPPEFWNKSMLEKPTDGRE VVCHASAWDFYNGKDFRIKQCTTVN LEDLVVAHHEMGHIQYFMQYKDLPV ALREGANPGFHEAIGDVLALSVSTP KHLHSLNLLSSEGGSDEHDINFLMK MALDKIAFIPFSYLVDQWRWRVFDG SITKENYNQEWWSLRLKYQGLCPPV PRTQGDFDPGAKFHIPSSVPYIRYF VSFIIQFQFHEALCQAAGHTGPLHK CDIYQSKEAGQRLATAMKLGFSRPW PEAMQLITGQPNMSASAMLSYFKPL LDWLRTENELHGEKLGWPQYNWTPN SARSEGPLPDSGRVSFLGLDLDAQQ AR
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -112 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: ACE angiotensin I converting enzyme [ Homo sapiens (human) ]
• Official Symbol: ACE
• Synonyms: ACE; angiotensin I converting enzyme; DCP; ACE1; DCP1; CD143; angiotensin-converting enzyme; CD143 antigen; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; carboxycathepsin; dipeptidyl carboxypeptidase 1; dipeptidyl carboxypeptidase I; kininase II; peptidase P; EC 3.4.15.1
• Gene ID: 1636
• mRNA Refseq: NM_000789
• Protein Refseq: NP_000780
• MIM: 106180
• UniProt ID: P12821