Recombinant Human ACTA2 protein, GST-tagged
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|Product Overview :||Recombinant Human ACTA2 protein(NP_001135417.1)(1-50 aa) was fused to GST Tag and expressed in E.coli.|
|Description :||This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.|
|Form :||The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.|
|Bio-activity :||Not tested.|
|Protein length :||1-50 aa|
|AA Sequence :||MCEEEDSTAL VCDNGSGLCK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG|
|Endotoxin :||Please contact the lab for more information.|
|Purity :||90%, by SDS-PAGE with Coomassie Brilliant Blue staining.|
|Applications :||Blocking peptide|
|Stability :||Store for up to 12 months at -20°C to -80°C as lyophilized powder.|
|Storage :||Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
|Reconstitution :||Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).|
|Gene Name :||ACTA2|
|Official Symbol :||ACTA2|
|Synonyms :||AAT6; ACTSA|
|Gene ID :||59|
|mRNA Refseq :||NM_001141945.2|
|Protein Refseq :||NP_001135417.1|
|UniProt ID :||P62736|
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