Active Recombinant Human ACVR2B Protein, His-tagged, Biotinylated

• Cat.No.: ACVR2B-886H
• Product Overview: Biotinylated Active Recombinant Human ACVR2B Protein(NP_001097.2) (Met 1-Thr 134) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-134 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1.Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2 µg/mL in the presence of 10 ng/mL recombinant Activin A. 2.Immobilized Recombinant Human/Mouse/Rat/Cynomolgus/Rhesus Activin A/INHBA Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human ACVR2B / ActivinR-IIB Protein (His Tag), Biotinylated, the EC50 is 2-8ng/mL.
• Molecular Mass: The recombinant Human ACVR2B comprises 127 amino acids and has a predicted molecular mass of 14.77 kDa. It migrates as an approximately 32.8 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ACVR2B activin A receptor, type IIB [ Homo sapiens ]
• Official Symbol: ACVR2B
• Synonyms: ACVR2B; activin A receptor, type IIB; activin receptor type-2B; ActR IIB; HTX4; ACTRIIB; ActR-IIB
• Gene ID: 93
• mRNA Refseq: NM_001106
• Protein Refseq: NP_001097
• MIM: 602730
• UniProt ID: Q13705