Recombinant Human ADAM8 protein, His-tagged

• Cat.No.: ADAM8-355H
• Product Overview: Recombinant Human ADAM8 protein (Ile17-Pro497), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Disintegrin and metalloproteinase domain-containing protein 8 is also known as ADAM8, Cell surface antigen MS2 and CD antigen CD156a, which belongs to the ADAM (a disintegrin and metalloprotease domain) family. ADAM family play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. ADAM8/CD156a contains 1 disintegrin domain, 1 EGF-like domain and 1 peptidase M12B domain. ADAM8 /MS2/CD156a is expressed on neutrophils and monocytes. ADAM8/MS2 possible involvement in extravasation of leukocytes.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, 20 mM CaCl2, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 53.8 kDa, which migrates as a 180-220 kDa band in DTT-reduced SDS-PAGE due to glycosylation. Two main fragments bands are visible with apparent MW of 69-73 kDa and 46-48 kDa respectively in reduced SDS-PAGE as a result of its being prone to proteolytic cleavage.
• Protein length: 481
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >85% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ADAM8
• Official Symbol: ADAM8
• Synonyms: ADAM8; ADAM metallopeptidase domain 8; a disintegrin and metalloproteinase domain 8; disintegrin and metalloproteinase domain-containing protein 8; CD156; MS2; cell surface antigen MS2; human leukocyte differentiation antigen; MGC134985
• Gene ID: 101
• mRNA Refseq: NM_001109
• Protein Refseq: NP_001100
• MIM: 602267
• UniProt ID: P78325