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Recombinant Human ADAR Mutant (K744R) Protein, Myc/DDK-tagged

Cat.No. : ADAR-127H
Product Overview : Purified mutant recombinant protein of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 4, mutation at(K744R) with a Myc/DDK tag was expressed in HEK293
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Description : This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 103.5 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for at least 12 months from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : > 50 μg/mL as determined by microplate Bradford method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Gene Name : ADAR adenosine deaminase RNA specific [ Homo sapiens (human) ]
Official Symbol : ADAR
Synonyms : ADAR; adenosine deaminase RNA specific; DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP; double-stranded RNA-specific adenosine deaminase; 136 kDa double-stranded RNA-binding protein; adenosine deaminase acting on RNA 1-A; dsRNA adenosine deaminase; dsRNA adeonosine deaminase; interferon-induced protein 4; interferon-inducible protein 4; EC 3.5.4.37
Gene ID : 103
mRNA Refseq : NM_001025107
Protein Refseq : NP_001020278
MIM : 146920
UniProt ID : P55265

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How could ADAR protein be used in gene therapy? 07/02/2022

ADAR protein could be used in gene therapy to correct mutations or disease-causing sequences in RNA molecules by introducing specific nucleotide changes. This could potentially restore normal RNA and protein function and treat or cure genetic diseases.

How is ADAR protein used in diagnostics? 07/14/2020

ADAR protein can be used as a biomarker for the diagnosis or prognosis of certain diseases. Dysregulation of ADAR activity has been implicated in various cancers and viral infections, and detection of ADAR in blood or tissue samples may aid in the diagnosis and prognosis of these diseases. Furthermore, RNA editing patterns may differ between healthy and diseased tissues, suggesting that changes in the RNA editing profile may be used as a diagnostic tool.

How is ADAR protein used in targeted therapies? 05/17/2020

ADAR protein can be targeted for therapies aimed at specific diseases or conditions. For example, targeting ADAR with small molecule inhibitors may be effective in treating certain cancers or viral infections. These therapies may work by selectively inhibiting ADAR-mediated RNA editing in cancer cells or the virus, leading to decreased tumor growth or viral replication. Additionally, ADAR may be targeted to modulate its activity in other diseases, such as autoimmune disorders. Development and optimization of ADAR-targeted therapies can be facilitated by studies using ADAR protein in vitro and in vivo.

Are there any known side effects of using ADAR protein for gene editing or therapy? 01/25/2020

It is not yet clear if there are any potential side effects of using ADAR protein for gene editing or therapy. One concern is off-target effects, where the protein may edit RNA at unintended sites, potentially leading to unintended consequences such as altering the function of proteins that are not targeted. Another concern is potential immune responses to the introduction of exogenous ADAR protein. However, much research is still needed to fully understand the safety of using ADAR-mediated gene editing or therapy.

Are there any limitations to the use of ADAR protein in gene editing or gene therapy? 10/03/2018

One limitation of ADAR-mediated RNA editing is its efficiency, as it can be difficult to achieve high levels of editing in target RNA molecules. Additionally, RNA editing can have unpredictable consequences on protein structure and function, which could lead to unintended effects. Finally, the delivery of ADAR protein and guide RNAs to target cells or tissues can be challenging and may require further optimization.

What are the advantages of using ADAR protein in gene editing compared to other gene editing tools? 09/15/2018

ADAR protein has the advantage of being able to modify RNA sequences without altering the underlying DNA sequence. This makes it a potentially safer alternative to DNA-based gene editing tools such as CRISPR/Cas9, which can introduce unintended changes in the DNA sequence. Additionally, ADAR protein can target specific RNA sequences and modify them in a site-specific manner, which could reduce off-target effects.

What are some potential applications of ADAR protein? 07/20/2018

ADAR protein has potential applications in gene editing and gene therapy, particularly for the treatment of genetic disorders such as cystic fibrosis, sickle cell anemia, and Huntington's disease. It may also have potential applications in cancer therapy, as well as in the study of RNA and its role in disease.

What are the potential applications of ADAR protein? 05/16/2017

The potential applications of ADAR protein include gene editing, drug discovery and development, diagnostics, and targeted therapies for various diseases, including cancer and viral infections. ADAR protein is involved in RNA editing, a process that modifies RNA molecules after they are transcribed from DNA. RNA editing has been shown to be important in regulating gene expression, and abnormal RNA editing has been implicated in disease development. Thus, targeting ADAR protein or its function may provide opportunities for new therapeutic approaches.

How is ADAR protein used in drug discovery and development? 04/16/2017

ADAR protein can be used in drug discovery and development by serving as a target for small molecule inhibitors or by modulating its activity in disease states. ADAR activity has been shown to be dysregulated in various cancers and viruses, making ADAR a potential drug target for these diseases. Small molecule inhibitors of ADAR may be developed to selectively inhibit its activity and prevent abnormal RNA editing in cancer or viral infections. Additionally, ADAR protein may be used in preclinical studies to evaluate the efficacy and safety of ADAR-targeted therapies.

Can ADAR protein be used to treat neurological disorders? 11/03/2016

There is potential for ADAR protein to be used to treat neurological disorders. ADAR is involved in the regulation of RNA metabolism, which is critical for proper neuronal function. Dysregulation of RNA editing has been implicated in the pathogenesis of several neurological disorders, including Huntington's, Parkinson's, and Alzheimer's diseases. ADAR-mediated RNA editing may represent a potential therapeutic target for these disorders by correcting aberrant protein function or expression. Additionally, assessing ADAR expression and RNA editing may also provide a diagnostic or prognostic biomarker for these disorders.

How is ADAR protein used in gene editing? 10/25/2016

ADAR protein can be used in gene editing by modifying RNA molecules. RNA editing changes the sequence of RNA by the insertion, deletion, or substitution of nucleotides. RNA editing can also modify splicing patterns, leading to the creation of new protein products. ADAR protein catalyzes the deamination of adenosine to inosine in RNA, and can be used to introduce specific changes in RNA sequences. RNA editing holds potential for correcting genetic defects or modifying gene expression in a variety of diseases, including cancer and genetic disorders.

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