Recombinant Human AhR, His-tagged

• Cat.No.: AhR-3644H
• Product Overview: Aryl Hydrocarbon Receptor (AhR)

Specification

• Description: This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons.
• Source: E. Coli
• Species: Human
• Tag: His
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 32.5kDa
• Protein length: 848
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of AhR recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: AHR aryl hydrocarbon receptor [ Homo sapiens ]
• Official Symbol: AhR
• Synonyms: AHR; aryl hydrocarbon receptor; bHLHe76; AH-receptor; ah receptor; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76
• Gene ID: 196
• mRNA Refseq: NM_001621
• Protein Refseq: NP_001612
• MIM: 600253
• UniProt ID: P35869
• Chromosome Location: 7p15
• Pathway: Adipogenesis, organism-specific biosystem
• Function: DNA binding; DNA binding; Hsp90 protein binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; protein binding; protein dimerization activity; protein heterodimerization activity; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; signal transducer activity; transcription factor binding; transcription regulatory region DNA binding

Reviews

09/01/2022

Thumbs up for this lab protein – it's top-notch!

02/13/2022

I'm loving this lab protein – it's a research game-changer.

07/14/2021

Five stars for this incredible lab protein – highly recommend.

08/07/2018

The lab protein's performance has exceeded our expectations, and its versatility has enabled diverse applications within our research scope.

06/13/2018

We've seen remarkable results with this lab protein.

Q&As

What are the genetic and epigenetic factors that regulate AHR expression and function?

The expression and function of AHR can be influenced by genetic polymorphisms, DNA methylation patterns, and histone modifications, which may have implications in individual susceptibility to environmental toxins.

How is AHR involved in skin biology and the response to environmental toxins?

AHR is expressed in skin cells and plays a role in the response to environmental toxins, UV radiation, and the regulation of skin barrier function.

What is the role of AHR in embryonic development and organogenesis?

AHR is essential for normal embryonic development and organogenesis. It participates in the patterning and differentiation of various organs, including the heart and vasculature.

Can AHR activation affect hormonal signaling pathways?

Yes, AHR activation can interfere with hormonal signaling pathways, leading to cross-talk with estrogen receptor signaling, and affecting hormonal homeostasis.

How does AHR influence cellular proliferation and apoptosis?

AHR can impact cell cycle progression and apoptosis by modulating the expression of genes involved in these processes, which can have implications in cancer biology.

What role does AHR play in the immune system and inflammation?

AHR is involved in the regulation of immune responses and inflammation by affecting the differentiation and function of immune cells, such as T cells and dendritic cells.

How does AHR contribute to xenobiotic metabolism and detoxification?

AHR activation induces the expression of cytochrome P450 enzymes, particularly CYP1A1 and CYP1B1, which metabolize and detoxify various environmental pollutants and xenobiotics.

What are the downstream targets of AHR signaling, and how is gene expression regulated by AHR?

AHR forms a complex with co-activators or co-repressors upon ligand binding, leading to the modulation of gene expression. Downstream targets include cytochrome P450 enzymes (CYP1A1, CYP1B1), phase II detoxification enzymes, and cytokines.

How does AHR activation occur, and what are its endogenous ligands?

AHR activation occurs upon binding to specific ligands, including polycyclic aromatic hydrocarbons (PAHs), halogenated aromatic hydrocarbons (HAHs), and tryptophan derivatives, such as indole-3-carbinol. Endogenous ligands are derived from cellular metabolism and the diet.

How is AHR activity regulated post-translationally, and what are the protein-protein interactions that modulate its function?

AHR activity can be regulated through various post-translational modifications, including phosphorylation and ubiquitination. Protein-protein interactions with chaperones and co-regulators also influence AHR function.