Recombinant Human AP2S1 293 Cell Lysate

• Cat.No.: AP2S1-8811HCL

Specification

• Description: Antigen standard for adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17delta is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: AP2S1 adaptor-related protein complex 2, sigma 1 subunit [ Homo sapiens ]
• Official Symbol: AP2S1
• Synonyms: AP2S1; adaptor-related protein complex 2, sigma 1 subunit; CLAPS2; AP-2 complex subunit sigma; sigma2-adaptin; HA2 17 kDa subunit; clathrin coat assembly protein AP17; clathrin coat-associated protein AP17; clathrin assembly protein 2 small chain; adaptor protein complex AP-2 subunit sigma; plasma membrane adaptor AP-2 17 kDa protein; adapter-related protein complex 2 sigma subunit; clathrin-associated/assembly/adaptor protein, small 2 (17kD); AP17; AP17-DELTA
• Gene ID: 1175
• mRNA Refseq: NM_021575
• Protein Refseq: NP_067586
• MIM: 602242
• UniProt ID: P53680
• Chromosome Location: 19q13.2-q13.3
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Disease, organism-specific biosystem; EGFR downregulation, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem; Endocytosis, organism-specific biosystem
• Function: protein transporter activity; transporter activity

Reviews

07/03/2021

Catalytic activity of AP2S1 is very strong, which can significantly improve the selectivity and yield of chemical reactions, and its application potential is huge.

06/16/2021

Experimenting with this recombinant protein allows me to focus more on experimental design and data analysis without worrying about the reproducibility of experimental results.

01/28/2021

Quality of AP2S1 is consistent, and we're reassured.

06/22/2019

Stability of AP2S1 is highly praised, and it can maintain its structural and functional stability even under various extreme environments, which is really impressive.

Q&As

Is the mutation of AP2S1 gene associated with hereditary diseases?

Genetic mutations in the AP2S1 have been shown to be associated with certain genetic diseases.

What is the structure of AP2S1 ?

The AP2S1 is a three-dimensional structure composed of multiple amino acid residues.

Which signaling pathways are associated with recombinant AP2S1?

AP2S1 is associated with various signaling pathways, such as EGFR signaling pathway and Notch signaling pathway.

Can AP2S1 be used as a diagnostic marker for tumors?

There is currently insufficient evidence to support recombinant AP2S1 as a diagnostic marker for tumors.

What is the function of AP2S1 ?

The AP2S1 is involved in protein breakdown and transport in the cell.

What is the significance of the research of AP2S1 for the development of new drugs?

The study of AP2S1 is of great significance for the research and development of new drugs, which can provide new ideas and directions for the discovery of drug targets.

How to detect the expression level of AP2S1 ?

Immunohistochemistry, Western blot and other methods can be used to detect the expression level of AP2S1 .

Is there any research on the mechanism of action of AP2S1 in tumorigenesis?

Some studies have shown that AP2S1 may play a role in tumorgenesis by influencing cell proliferation and metastasis.

Does the study of recombinant AP2S1 involve gene therapy?

There are currently no gene therapy studies involving recombinant AP2S1.

What is the application prospect of AP2S1 in tumor therapy?

Recombinant protein AP2S1 may be a potential target for tumor therapy, but further research and validation are needed.

Is there any research on the interaction of AP2S1 with drugs?

There has not been much research on the interaction of AP2S1 with drugs.

Can AP2S1 be used as a drug target?

The AP2S1 may be one of the targets for drug development, but further studies are needed to confirm it.

What is the distribution of AP2S1 in human body?

The AP2S1 is widely distributed in the human body, including the nervous system and several organs.

In what diseases does recombinant AP2S1 play a key role?

The AP2S1 plays a key role in many diseases such as nervous system diseases and tumors.

What is the progress of drug development targeting AP2S1 ?

The current drug development targeting AP2S1 is still in the preliminary stage, and further research and development is needed.

Is the association between recombinant AP2S1 and disease genetic?

The association of recombinant AP2S1 with certain diseases may have genetic characteristics.

In which cell types is AP2S1 expressed?

Recombinant AP2S1 protein is expressed in a variety of cell types.

Is there any research on the function of AP2S1 in neurological diseases?

Some studies have shown that recombinant AP2S1 may be involved in regulating synaptic transmission and other functions in nervous system diseases.

Can recombinant AP2S1 be used as a therapeutic target for this disease?

The AP2S1 may be a potential target for the treatment of this disease, but further studies are needed to confirm it.