Recombinant Human APOE Protein, His-tagged

• Cat.No.: APOE-56H
• Product Overview: Recombinant Human Apolipoprotein E is produced by our Mammalian expression system and the target gene encoding Lys19--‐His317 is expressed with a 6His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Description: Apolipoprotein E (ApoE) is a glycoprotein that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. ApoE can be synthesized by the liver and intestinally synthesized chylomicrons. ApoE mediates high-affinity binding of chylomicrons and very low density lipoprotein (VLDL) particles to the LDL receptor, allowing for specific uptake of these particles by the liver, preventing the accumulation of cholesterol-rich particles in the plasma. ApoE plays an essential role in the formation of plasma lipoproteins and is involved in their production, conversion and clearance. ApoE is also a constituent of a subclass of high density of lipoproteins (HDL) involved in cholesterol transport.
• Form: Lyophilized frum a 0.2 um filtered solution of 2umM PBS, 5mM CHAPS, 2mM DDT, pH7.4.
• Molecular Mass: Predicted Molecular Weight: 34.9kDa
  Apparent Molecular Weight: 35-45kDa, reducing conditions.
• AA Sequence: Lys19-His317
• Endotoxin: Less than 0.1 EU/ug.
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: APOE apolipoprotein E [ Homo sapiens (human) ]
• Official Symbol: APOE
• Synonyms: AD2; LPG; APO-E; ApoE4; LDLCQ5; apolipoprotein E; apolipoprotein E3
• Gene ID: 348
• mRNA Refseq: NM_000041.4
• Protein Refseq: NP_000032.1
• MIM: 107741
• UniProt ID: P02649