Recombinant Human BACE1 protein, His & GST-tagged

• Cat.No.: BACE1-713H
• Product Overview: Recombinant Human BACE1 aa. (Phe170~Thr428 (Accession# P56817)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 59.1kDa
• Protein length: Phe170~Thr428 (Accession# P56817)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 5.2.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: BACE1 beta-secretase 1 [ Homo sapiens (human) ]
• Official Symbol: BACE1
• Synonyms: BACE1; beta-secretase 1; ASP2; BACE; HSPC104; beta-secretase 1; APP beta-secretase; asp 2; aspartyl protease 2; beta-secretase 1 precursor variant 1; beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme; beta-site amyloid beta A4 precursor protein-cleaving enzyme; memapsin-2; membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2
• Gene ID: 23621
• mRNA Refseq: NM_001207049.1
• Protein Refseq: NP_001193978.1
• UniProt ID: P56817