| Description : |
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway. |
| Source : |
Wheat Germ |
| Species : |
Human |
| Tag : |
GST |
| Form : |
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
| Molecular Mass : |
95.3 kDa |
| AA Sequence : |
MDPAEAVLQEKALKFMCSMPRSLWL GCSSLADSMPSLRCLYNPGTGALTA FQNSSEREDCNNGEPPRKIIPEKNS LRQTYNSCARLCLNQETVCLASTAM KTENCVAKTKLANGTSSMIVPKQRK LSASYEKEKELCVKYFEQWSESDQV EFVEHLISQMCHYQHGHINSYLKPM LQRDFITALPARGLDHIAENILSYL DAKSLCAAELVCKEWYRVTSDGMLW KKLIERMVRTDSLWRGLAERRGWGQ YLFKNKPPDGNAPPNSFYRALYPKI IQDIETIESNWRCGRHSLQRIHCRS ETSKGVYCLQYDDQKIVSGLRDNTI KIWDKNTLECKRILTGHTGSVLCLQ YDERVIITGSSDSTVRVWDVNTGEM LNTLIHHCEAVLHLRFNNGMMVTCS KDRSIAVWDMASPTDITLRRVLVGH RAAVNVVDFDDKYIVSASGDRTIKV WNTSTCEFVRTLNGHKRGIACLQYR DRLVVSGSSDNTIRLWDIECGACLR VLEGHEELVRCIRFDNKRIVSGAYD GKIKVWDLVAALDPRAPAGTLCLRT LVEHSGRVFRLQFDEFQIVSSSHDD TILIWDFLNDPAAQAEPPRSPSRTY TYISR |
| Quality Control Test : |
12.5% SDS-PAGE Stained with Coomassie Blue. |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : |
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
