Recombinant Human CA14 Protein, His-tagged

• Cat.No.: CA14-118H
• Product Overview: Recombinant human CA14 protein with His tag was expressed in HEK293.

Specification

• Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: 31.6 kDa
• Protein Length: 337
• AA Sequence: MLFSALLLEVIWILAADGGQHWTYE GPHGQDHWPASYPECGNNAQSPIDI QTDSVTFDPDLPALQPHGYDQPGTE PLDLHNNGHTVQLSLPSTLYLGGLP RKYVAAQLHLHWGQKGSPGGSEHQI NSEATFAELHIVHYDSDSYDSLSEA AERPQGLAVLGILIEVGETKNIAYE HILSHLHEVRHKDQKTSVPPFNLRE LLPKQLGQYFRYNGSLTTPPCYQSV LWTVFYRRSQISMEQLEKLQGTLFS TEEEPSKLLVQNYRALQPLNQRMVF ASFIQAGSSYTTGEMLSLGVGILVG CLCLLLAVYFIARKIRKKRLENRKS VVFTSAQATTEA
• Purity: > 98%
• Applications: WB; ELISA; FACS; FC
• Stability: This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use.
• Storage: At -20 centigrade.
• Concentration: 1 mg/mL
• Storage Buffer: PBS (pH 7.4-7.5). Sterile-filtered colorless solution.
• Reconstitution: Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.

Gene Information

• Gene Name: CA14 carbonic anhydrase XIV [ Homo sapiens (human) ]
• Official Symbol: CA14
• Synonyms: CA14; carbonic anhydrase XIV; carbonic anhydrase 14; CA-XIV; carbonic dehydratase; carbonate dehydratase XIV; CAXiV
• Gene ID: 23632
• mRNA Refseq: NM_012113
• Protein Refseq: NP_036245
• MIM: 604832
• UniProt ID: Q9ULX7

Reviews

08/19/2022

Reliable source, ensures reproducibility in our research.

11/10/2020

Efficient services, optimize our protein-related studies.

10/01/2020

Responsive customer support, aids in problem-solving effectively.

Q&As

Are there any known small molecule modulators specific to CA14's activity?

Specific modulators can be identified through high-throughput screening assays targeting CA14 and validated using in vitro and in vivo models.

What unique functional attributes differentiate CA14 from other carbonic anhydrase isoforms?

CA14's unique functional attributes can be discerned using comparative enzymatic assays and structural analyses against other isoforms.

How do changes in cellular pH or ion concentrations influence CA14 activity?

Influence of pH or ions on CA14 can be analyzed using enzymatic assays under varying conditions and ion-sensitive probes.

Are there any known disease associations where CA14 mutations or dysregulation play a pivotal role?

Disease associations can be determined through genetic studies, patient tissue analyses, and literature reviews focusing on CA14's relevance in pathological conditions.

In what tissues or cell types is CA14 predominantly expressed, and how does this correlate with its physiological role?

Predominant tissue or cell-type expression can be determined using tissue microarrays, qPCR, or proteomic databases, correlating findings with potential physiological implications.

What are the consequences of CA14 overexpression or knockdown in cellular and organismal models?

Consequences of overexpression or knockdown can be assessed using phenotypic analyses, cellular viability assays, and in vivo studies in organisms with altered CA14 expression.

Are there specific cellular pathways where CA14 plays a crucial modulatory role?

CA14's role in cellular pathways can be explored using pathway enrichment analyses, proteomic studies, and pathway-specific assays in CA14-modified cells.