Recombinant Human CA5A, His tagged

• Cat.No.: CA5A-1142H
• Product Overview: Recombinant Human CA5A (NP_001730.1) mature form (Ala 40-Ser 305), fused with an Met at N-terminus and a polyhistide tag at the C-terminus, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50mM NaAc, 50mM NaCl, 0.05% Brij 35, pH 5.0
• Molecular Mass: The recombinant human CA5A consisting of 277 amino acids and has a calculated molecular mass of 31.6 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CA5A carbonic anhydrase VA, mitochondrial [ Homo sapiens ]
• Official Symbol: CA5A
• Gene ID: 763
• mRNA Refseq: NM_001739
• Protein Refseq: NP_001730
• MIM: 114761
• UniProt ID: P35218

Reviews

08/26/2022

Efficient protein quantification, saves time and resources.

01/15/2022

Consistency in results, a symbol of their service quality.

05/25/2021

Top-tier protein sequencing, propels our research forward.

Q&As

What post-translational modifications are observed in CA5A?

Post-translational modifications of CA5A can be identified using mass spectrometry, with potential phosphorylation or acetylation sites.

What diseases or conditions are associated with CA5A dysregulation?

Dysregulation of CA5A might influence metabolic processes in the liver, potentially impacting conditions like fatty liver disease or other metabolic disorders.

Are there genetic mutations in the CA5A gene that impact its function?

Specific mutations might affect CA5A's stability or activity, necessitating functional assays for validation.

Are there specific compounds known to inhibit or activate CA5A activity?

While general carbonic anhydrase inhibitors might affect CA5A, specific modulators would need further research.

What is the primary function of CA5A in cellular processes?

CA5A facilitates the reversible conversion of CO2 to bicarbonate and protons, playing a role in pH regulation and CO2 transport.

How does CA5A's enzymatic activity compare with other carbonic anhydrase isoforms?

CA5A's activity is tailored to its mitochondrial environment, which might differ in kinetics from cytosolic isoforms.

How does the expression profile of CA5A differ among human tissues?

CA5A is primarily expressed in the mitochondria of liver cells, with lesser amounts in other tissues.