Recombinant Human CA7 293 Cell Lysate

• Cat.No.: CA7-7913HCL

Specification

• Description: Antigen standard for carbonic anhydrase VII (CA7), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: CA7 carbonic anhydrase VII [ Homo sapiens ]
• Official Symbol: CA7
• Synonyms: CA7; carbonic anhydrase VII; carbonic anhydrase 7; CA-VII; carbonic dehydratase VII; carbonate dehydratase VII; CAVII
• Gene ID: 766
• mRNA Refseq: NM_001014435
• Protein Refseq: NP_001014435
• MIM: 114770
• UniProt ID: P43166
• Chromosome Location: 16q22.1
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

Reviews

09/20/2022

Expertise in protein analysis, accelerates our research.

07/13/2021

Affordable pricing, excellent value for research budgets.

05/22/2021

Invaluable for our protein-related discoveries and innovations.

Q&As

What distinguishes the enzymatic function of CA7 from other carbonic anhydrase family members?

CA7 facilitates the reversible hydration of carbon dioxide, but its specific kinetics might differ from other isoforms.

How does CA7 contribute to cellular pH balance and metabolism?

CA7 aids in CO2 transport and pH regulation, playing a crucial role in metabolic processes.

Have any diseases or conditions been associated with aberrant CA7 expression or activity?

Dysregulation or mutations in CA7 might influence metabolic balance in tissues, potentially impacting conditions related to liver function or metabolism.

Are there compounds known to specifically inhibit CA7 activity?

While some general carbonic anhydrase inhibitors might affect CA7, isoform-specific inhibitors would require further research.

In which specific human tissues is CA7 expression most prevalent?

CA7 is primarily expressed in the liver, among other tissues.

What post-translational modifications are characteristic of CA7?

Potential modifications like phosphorylation or acetylation can be identified using techniques like mass spectrometry.

Are there any genetic mutations in the CA7 gene known to influence its enzymatic functionality?

Specific mutations could alter CA7's enzyme kinetics or stability, requiring in vitro functional assays for verification.