Active Recombinant Human CCL20 protein, His-tagged

• Cat.No.: CCL20-161H
• Product Overview: Active Recombinant Human CCL20 protein(P78556)(27~96 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 27-96 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Macrophage Inflammatory Protein 3 Alpha (MIP-3α), also known as LARC (liver and activation-regulated chemokine), Exodus-1 or CCL20, is a CC chemokine with a selective chemotactic activity for lymphocytes and dendritic cells (DCs). MIP3α is produced by activated cells, including monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts and is expressed in liver, lung, and some lymphoid tissues. This chemokine elicits its effects on its target cells by binding to the chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells, CCR7 central-memory T-Cells. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of recombinant human MIP-3α on the Jurkat cell line. Briefly, Jurkat cells were seeded into the upper chambers (200μL cell suspension,10^6 cells/mL in RPMI 1640 with FBS free) and MIP-3α (0.01ng/mL, 0.1ng/mL, 1ng/mL, 10ng/mL,100ng/mL and 1000ng/mL diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8 μm pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows MIP-3α is able to induce migration of Jurkat cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). The optimum chemotaxis of recombinant human MIP-3α occurs at 1-10ng/mL.
• Molecular Mass: 9.3kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CCL20 chemokine (C-C motif) ligand 20 [ Homo sapiens ]
• Official Symbol: CCL20
• Synonyms: CCL20; chemokine (C-C motif) ligand 20; SCYA20, small inducible cytokine subfamily A (Cys Cys), member 20; C-C motif chemokine 20; CKb4; exodus 1; LARC; MIP 3a; ST38; exodus-1; MIP-3-alpha; CC chemokine LARC; beta chemokine exodus-1; beta-chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20; MIP3A; MIP-3a; SCYA20
• Gene ID: 6364
• mRNA Refseq: NM_001130046
• Protein Refseq: NP_001123518
• MIM: 601960
• UniProt ID: P78556