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Recombinant Human CD209 cell lysate

Cat.No. : CD209-2986HCL
Product Overview : Human CD209 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CD209 CD209 molecule [ Homo sapiens ]
Official Symbol : CD209
Synonyms : CD209; CD209 molecule; CD209 antigen; CDSIGN; CLEC4L; DC SIGN; DC SIGN1; HIV gpl20-binding protein; C-type lectin domain family 4 member L; C-type lectin domain family 4, member L; dendritic cell-specific ICAM-3-grabbing non-integrin 1; dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin; DC-SIGN; DC-SIGN1; MGC129965;
Gene ID : 30835
mRNA Refseq : NM_001144893
Protein Refseq : NP_001138365
MIM : 604672
UniProt ID : Q9NNX6
Chromosome Location : 19p13
Pathway : Measles, organism-specific biosystem; Measles, conserved biosystem; Phagosome, organism-specific biosystem; Phagosome, conserved biosystem; Tuberculosis, organism-specific biosystem; Tuberculosis, conserved biosystem;
Function : mannose binding; metal ion binding; peptide antigen binding; receptor activity; sugar binding; virion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can you discuss the role of CD209 in shaping adaptive immune responses and its impact on immunotherapy strategies? 01/23/2021

CD209 influences adaptive immune responses by modulating dendritic cell-T cell interactions. Understanding its role is critical for optimizing immunotherapy approaches, particularly in vaccine development.

How does CD209 participate in the recognition of glycosylated pathogens, and what are the implications for innate immunity? 11/14/2019

CD209 recognizes glycosylated patterns on pathogens, particularly through interactions with high-mannose structures. This recognition is crucial for initiating innate immune responses.

Can you elaborate on the genetic polymorphisms of CD209 and their association with susceptibility to infectious diseases? 06/09/2019

Genetic polymorphisms in CD209 have been associated with susceptibility to infectious diseases, including tuberculosis and HIV, suggesting a role in modulating host-pathogen interactions.

How does CD209 contribute to the formation of immunological synapses between dendritic cells and T cells, and what molecular events are involved? 02/09/2019

CD209 facilitates immunological synapse formation by mediating adhesion between dendritic cells and T cells. Molecular events involve interactions with ICAM-3 and other adhesion molecules.

How does CD209 contribute to immune responses, particularly in the context of antigen presentation? 06/02/2018

CD209 facilitates antigen capture by dendritic cells and modulates immune responses by mediating the interaction between dendritic cells and T cells during antigen presentation.

Customer Reviews (3)

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Reviews
01/07/2021

    The team promptly addressed my inquiries and provided valuable assistance in selecting the right recombined protein for our research.

    12/23/2019

      This product consistently delivers accurate results in our experiments.

      09/14/2018

        Quick service! The team provided efficient assistance in choosing the right protein for us.

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