||Recombinant Human CD274 Protein (NP_054862.1, Met1-Arg238) was expressed in HEK293 cells.
||Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
||Lyophilized from sterile PBS, pH 7.4.
||The recombinant human CD274 consists of 220 amino acids and predicts a molecular mass of 25.2 kDa.
||< 1.0 EU per μg protein as determined by the LAL method.
||> 95 % as determined by SDS-PAGE.
||Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
||Reconstitute in ddH2O.