Active Recombinant Human CD4 protein, hFc-tagged, Low Endotoxin

• Cat.No.: CD4-052H
• Product Overview: Active Recombinant Human CD4 protein(P01730)(Lys26-Trp390) is expressed from HEK293 with hFc (IgG1) at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 26-390 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS, 100mM L-arginine (pH 7.4).
• Bio-activity: Immobilized Human CD4 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 67.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD4 CD4 molecule [ Homo sapiens ]
• Official Symbol: CD4
• Synonyms: CD4; CD4 molecule; CD4 antigen (p55) , T cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4mut
• Gene ID: 920
• mRNA Refseq: NM_000616
• Protein Refseq: NP_000607
• MIM: 186940
• UniProt ID: P01730