Recombinant Human CD40 Ligand Protein, His-tagged CD40L-326H

Recombinant Human CD40 Ligand Protein, His-tagged

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Recombinant Human CD40 Ligand Protein, His-tagged

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Cat.No. : CD40L-326H
Product Overview : Recombinant Human CD40L (NP_000065.1) (Glu108-Leu261) was expressed with an initial Met at the N-terminus and a polyhistide tag at the C-terminus. The purified protein was biotinylated in vitro.
Description : The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which ass centigradeiating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal pr centigradeess but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen- presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.
Source : E. coli
Species : Human
Tag : His
Predicted N Terminal : Met
Form : Lyophilized from sterile PBS,pH7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized human CD40-His at 10μg/mL (100μL/well) can bind biotinylated human CD40L, The EC50 of biotinylated human CD40L is 0.08-0.2μg/mL.
Molecular Mass : The recombinant human CD40L consists of 161 amino acids and has a calculated molecular mass of 17.7 kDa.
Protein length : Glu108-Leu261
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95% as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Official Symbol : CD40L
Synonyms : CD40L;IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L;CD40 ligand;CD40 antigen ligand;CD40-L;T-B cell-activating molecule;T-cell antigen Gp39;TNF-related activation protein;tumor necrosis factor (ligand) superfamily member 5

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