Recombinant Human CD63 protein, His-tagged

• Cat.No.: CD63-3890H
• Product Overview: Recombinant Human CD63 protein(P08962-1)(Ala103-Val203), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CD63 consists of 112 amino acids after removal of the signal peptide and has a predicted molecular mass of 13 kDa. The apparent molecular mass of rh CD63 is approximately 22.8 kDa in SDS-PAGE under reducing conditions.
• Protein length: Ala103-Val203
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD63 CD63 molecule [ Homo sapiens ]
• Official Symbol: CD63
• Synonyms: CD63; CD63 molecule; CD63 antigen (melanoma 1 antigen) , MLA1; CD63 antigen; ME491; TSPAN30; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); melanoma-associated antigen ME491; ocular melanoma-associated antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; MLA1; LAMP-3; OMA81H
• Gene ID: 967
• mRNA Refseq: NM_001257389
• Protein Refseq: NP_001244318
• MIM: 155740
• UniProt ID: P08962

Citation

Efficient Payload Delivery by a Bispecific Antibody–Drug Conjugate Targeting HER2 and CD63

Journal: Molecular Cancer Therapeutics    Data: 2016/8/24

Authors: Bart E.C.G. de Goeij, Tom Vink, Paul W.H.I. Parren

Article Snippet:The plates were blocked with 2% chicken serum and incubated with 1 μg/mL histidine- 6 mutated IgG1-2192.The plates were blocked with 2% chicken serum and incubated with 1 μg/mL histidine- 6 mutated IgG1-2192.. Serially diluted (1-0.0005 μg/mL) recombinant human CD63 (Creative BioMart) was added followed by 1 μg/mL mouse anti-poly-histidine-biotin (R&D).. The reaction was visualized using ABTS and stopped with oxalic acid.The reaction was visualized using ABTS and stopped with oxalic acid.

Publication :

Efficient payload delivery by a bispecific antibody targeting HER2 and CD63 (2016)

Reviews

11/26/2022

Consistent and reliable protein.

08/23/2022

Excellent yield, very pure.

06/19/2021

Perfect for specific experiments.

Q&As

How is CD63 involved in cancer progression?

It is associated with cancer progression, impacting cell migration and metastasis.

What role does CD63 play in immune cell function?

CD63 modulates immune responses, influencing antigen presentation and cell activation.

Are there potential therapeutic applications targeting CD63?

Targeting CD63 may offer therapeutic potential in cancer and immune-related diseases.

How is CD63 expression regulated in cells?

Its expression is regulated through transcriptional and post-translational mechanisms.

How does CD63 contribute to vesicular trafficking?

It helps in the sorting and transport of proteins within endosomes and lysosomes.

What is the relationship between CD63 and exosomes?

CD63 is a marker for exosomes, playing a role in their formation and function.

What is the primary function of CD63 in cellular processes?

CD63 is involved in vesicular trafficking, cell adhesion, and signaling.