Recombinant Human CD63 protein, His-tagged

• Cat.No.: CD63-3890H
• Product Overview: Recombinant Human CD63 protein(P08962-1) (Ala 103-Val 203) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 103-203 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human CD63 consists of 112 amino acids after removal of the signal peptide and has a predicted molecular mass of 13 kDa. The apparent molecular mass of rh CD63 is approximately 20-25 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Efficient payload delivery by a bispecific antibody targeting HER2 and CD63 (2016)

Gene Information

• Gene Name: CD63 CD63 molecule [ Homo sapiens ]
• Official Symbol: CD63
• Synonyms: CD63; CD63 molecule; CD63 antigen (melanoma 1 antigen), MLA1; CD63 antigen; ME491; TSPAN30; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); melanoma-associated antigen ME491; ocular melanoma-associated antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; MLA1; LAMP-3; OMA81H
• Gene ID: 967
• mRNA Refseq: NM_001257389
• Protein Refseq: NP_001244318
• MIM: 155740
• UniProt ID: P08962

Citation

Efficient Payload Delivery by a Bispecific Antibody–Drug Conjugate Targeting HER2 and CD63

Journal: Molecular Cancer Therapeutics    Data: 2016/8/24

Authors: Bart E.C.G. de Goeij, Tom Vink, Paul W.H.I. Parren

Article Snippet:The plates were blocked with 2% chicken serum and incubated with 1 μg/mL histidine- 6 mutated IgG1-2192.The plates were blocked with 2% chicken serum and incubated with 1 μg/mL histidine- 6 mutated IgG1-2192.. Serially diluted (1-0.0005 μg/mL) recombinant human CD63 (Creative BioMart) was added followed by 1 μg/mL mouse anti-poly-histidine-biotin (R&D).. The reaction was visualized using ABTS and stopped with oxalic acid.The reaction was visualized using ABTS and stopped with oxalic acid.