Recombinant Sus scrofa (Pig) CD63 cell lysate

• Cat.No.: CD63-1553SCL
• Product Overview: Sus scrofa (Pig) CD63 / Tspan-30 / Tetraspanin-30 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Sus scrofa (Pig)
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: CD63 CD63 molecule [ Sus scrofa ]
• Official Symbol: CD63
• Synonyms: CD63 molecule; CD63 antigen
• Gene ID: 100155929
• mRNA Refseq: XM_005663878
• Protein Refseq: XP_005663935
• Chromosome Location: 5; 5
• Pathway: Hemostasis, organism-specific biosystem; Lysosome, organism-specific biosystem

Reviews

11/26/2022

Consistent and reliable protein.

08/23/2022

Excellent yield, very pure.

06/19/2021

Perfect for specific experiments.

Q&As

How is CD63 involved in cancer progression?

It is associated with cancer progression, impacting cell migration and metastasis.

What role does CD63 play in immune cell function?

CD63 modulates immune responses, influencing antigen presentation and cell activation.

Are there potential therapeutic applications targeting CD63?

Targeting CD63 may offer therapeutic potential in cancer and immune-related diseases.

How is CD63 expression regulated in cells?

Its expression is regulated through transcriptional and post-translational mechanisms.

How does CD63 contribute to vesicular trafficking?

It helps in the sorting and transport of proteins within endosomes and lysosomes.

What is the relationship between CD63 and exosomes?

CD63 is a marker for exosomes, playing a role in their formation and function.

What is the primary function of CD63 in cellular processes?

CD63 is involved in vesicular trafficking, cell adhesion, and signaling.