Recombinant Human CHUK Protein, GST-tagged

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Recombinant Human CHUK Protein, GST-tagged

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Cat.No. : CHUK-5129H
Product Overview : Human CHUK (NP_001269.3, 1 a.a. - 745 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Description : This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq
Source : Insect Cell
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization).
Substrate: I-kappaB-alpha peptide.
ATP: 100 μM.
Molecular Mass : 111 kDa
Purity : 93 % by SDS-PAGE/CBB staining
Applications : Functional Study
SDS-PAGE
Storage : Store at -8 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : CHUK conserved helix-loop-helix ubiquitous kinase [ Homo sapiens ]
Official Symbol : CHUK
Synonyms : CHUK; conserved helix-loop-helix ubiquitous kinase; TCF16; inhibitor of nuclear factor kappa-B kinase subunit alpha; IkBKA; IKK alpha; IKK1; IKKA; NFKBIKA; TCF-16; IKK-a kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha; IKBKA; IKK-alpha;
Gene ID : 1147
mRNA Refseq : NM_001278
Protein Refseq : NP_001269
MIM : 600664
UniProt ID : O15111

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