Recombinant Human CHUK Protein, GST-tagged
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|Product Overview :||Human CHUK (NP_001269.3, 1 a.a. - 745 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.|
|Description :||This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq|
|Source :||Insect Cell|
|Bio-activity :||The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization).
Substrate: I-kappaB-alpha peptide.
ATP: 100 μM.
|Molecular Mass :||111 kDa|
|Purity :||93 % by SDS-PAGE/CBB staining|
|Applications :||Functional Study
|Storage :||Store at -8 centigrade. Aliquot to avoid repeated freezing and thawing.|
|Storage Buffer :||In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)|
|Quality Control Testing :||
Loading 1 μg protein in SDS-PAGE
|Result of activity analysis :||
|Gene Name :||CHUK conserved helix-loop-helix ubiquitous kinase [ Homo sapiens ]|
|Official Symbol :||CHUK|
|Synonyms :||CHUK; conserved helix-loop-helix ubiquitous kinase; TCF16; inhibitor of nuclear factor kappa-B kinase subunit alpha; IkBKA; IKK alpha; IKK1; IKKA; NFKBIKA; TCF-16; IKK-a kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha; IKBKA; IKK-alpha;|
|Gene ID :||1147|
|mRNA Refseq :||NM_001278|
|Protein Refseq :||NP_001269|
|UniProt ID :||O15111|
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