Recombinant Human CSH1 Protein, His-tagged

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Recombinant Human CSH1 Protein, His-tagged

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Cat.No. : CSH1-037H
Product Overview : Recombinant human CSH1, fused to His-tag at Cterminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description : The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, although the ratio of 1 to 2 increases by term. Mutations in this gene result in placental lactogen deficiency and Silver-Russell syndrome.
Source : Insect cells
Species : Human
Tag : His
Form : Liquid
Bio-activity : Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.8 ng/mL.
Molecular Mass : 23.1 kDa
AA Sequence : VQTVPLSRLF DHAMLQAHRA HQLAIDTYQE FEETYIPKDQ KYSFLHDSQT SFCFSDSIPT PSNMEETQQK SNLELLRISL LLIESWLEPV RFLRSMFANN LVYDTSDSDD YHLLKDLEEG IQTLMGRLED GSRRTGQILK QTYSKFDTNS HNHDALLKNY GLLYCFRKDM DKVETFLRMV QCRSVEGSCG F<HHHHHH>
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 90% by SDS-PAGE
Applications : SDS-PAGE, Bioactivity
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -116 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/mL (determined by Absorbance at 280nm)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol
SDS-PAGE :

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity :

Human CSH1 stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.8 ng/mL.

Gene Name : CSH1 chorionic somatomammotropin hormone 1 [ Homo sapiens (human) ]
Official Symbol : CSH1
Synonyms : CSH1; chorionic somatomammotropin hormone 1; PL; CSA; CS-1; CSMT; GHB3; hCS-1; hCS-A; chorionic somatomammotropin hormone 1; Chorionic somatomammotropin hormone 2; choriomammotropin; chorionic somatomammotropin A; chorionic somatomammotropin-1; growth hormone B3; placental lactogen
Gene ID : 1442
mRNA Refseq : NM_001317
Protein Refseq : NP_001308
MIM : 150200
UniProt ID : P0DML2

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