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Recombinant Human CTSB protein, His-tagged

Cat.No. : CTSB-385H
Product Overview : Recombinant Human CTSB protein (Arg18-Ile339), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B/CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B/ APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B/CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 36.7 kDa (pro-form) and 29.0 kDa (mature-form). Due to glycosylation, The reducing (R) protein migrates as 42-50 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band.
Protein length : 322
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CTSB-385H :

Human Cathepsin B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : CTSB
Official Symbol : CTSB
Synonyms : CTSB; cathepsin B; cathepsin B1; APP secretase; cysteine protease; amyloid precursor protein secretase; APPS; CPSB
Gene ID : 1508
mRNA Refseq : NM_001908
Protein Refseq : NP_001899
MIM : 116810
UniProt ID : P07858

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