Recombinant Human CYP2E1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: CYP2E1-430H
• Product Overview: CYP2E1 MS Standard C13 and N15-labeled recombinant protein (NP_000764) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 56.7 kDa
• AA Sequence: MSALGVTVALLVWAAFLLLVSMWRQ VHSSWNLPPGPFPLPIIGNLFQLEL KNIPKSFTRLAQRFGPVFTLYVGSQ RMVVMHGYKAVKEALLDYKDEFSGR GDLPAFHAHRDRGIIFNNGPTWKDI RRFSLTTLRNYGMGKQGNESRIQRE AHFLLEALRKTQGQPFDPTFLIGCA PCNVIADILFRKHFDYNDEKFLRLM YLFNENFHLLSTPWLQLYNNFPSFL HYLPGSHRKVIKNVAEVKEYVSERV KEHHQSLDPNCPRDLTDCLLVEMEK EKHSAERLYTMDGITVTVADLFFAG TETTSTTLRYGLLILMKYPEIEEKL HEEIDRVIGPSRIPAIKDRQEMPYM DAVVHEIQRFITLVPSNLPHEATRD TIFRGYLIPKGTVVVPTLDSVLYDN QEFPDPEKFKPEHFLNENGKFKYSD YFKPFSTGKRVCAGEGLARMELFLL LCAILQHFNLKPLVDPKDIDLSPIH IGFGCIPPRYKLCVIPRSTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CYP2E1 cytochrome P450 family 2 subfamily E member 1 [ Homo sapiens (human) ]
• Official Symbol: CYP2E1
• Synonyms: CYP2E1; cytochrome P450 family 2 subfamily E member 1; CPE1; CYP2E; P450-J; P450C2E; cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; cytochrome P450-J; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; EC 1.14.13.n7; EC 1.14.14.1
• Gene ID: 1571
• mRNA Refseq: NM_000773
• Protein Refseq: NP_000764
• MIM: 124040
• UniProt ID: P05181