Recombinant Human DPM1 Protein, His-Tagged

• Cat.No.: DPM1-01H
• Product Overview: Recombinant Human DPM1 Protein, His-tagged was expressed in E.coli cell

Specification

• Description: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants.
• Source: Escherichia coli
• Species: Human
• Tag: His
• Molecular Mass: The protein has a calculated MW of 30.45 kDa.
• AA Sequence: HHHHHHMASLEVSRSPRRSR RELEVRSPRQ NKYSVLLPTYNERENLPLIV WLLVKSFSESGINYEIIIID DGSPDGTRDVAEQLEKIYGSDRILL RPREK KLGLGTAYIHGMKHATGNYIIIMDA DLSHHPKFIPEFIRKQKEGNFDIVS GTRYKGNGGVYGWDLKRKIISRGAN FLTQILLRPGASDLTGSFRLYRKEV LEKLIEKCVSKGYVFQMEMIVRARQ LNYTIGEVPISFVDRVYGESKLGGN EIVSFLKGLLTLFATT
• Purity: >90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.4 mg/mL
• Storage Buffer: PBS, pH 7.4

Gene Information

• Gene Name: DPM1 dolichyl-phosphate mannosyltransferase subunit 1, catalytic [ Homo sapiens (human) ]
• Official Symbol: DPM1
• Synonyms: MPDS; CDGIE
• Gene ID: 8813
• mRNA Refseq: NM_001317034.1
• Protein Refseq: NP_001303963.1
• MIM: 603503
• UniProt ID: O60762