Recombinant Human EGFR Protein, His-tagged

• Cat.No.: EGFR-211H
• Product Overview: Recombinant human EGFR protein with His tag was expressed in HEK293.

Specification

• Description: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: 69.5 kDa
• Protein length: 1210
• AA Sequence: MRPSGTAGAALLALLAALCPASRAL EEKKVCQGTSNKLTQLGTFEDHFLS LQRMFNNCEVVLGNLEITYVQRNYD LSFLKTIQEVAGYVLIALNTVERIP LENLQIIRGNMYYENSYALAVLSNY DANKTGLKELPMRNLQEILHGAVRF SNNPALCNVESIQWRDIVSSDFLSN MSMDFQNHLGSCQKCDPSCPNGSCW GAGEENCQKLTKIICAQQCSGRCRG KSPSDCCHNQCAAGCTGPRESDCLV CRKFRDEATCKDTCPPLMLYNPTTY QMDVNPEGKYSFGATCVKKCPRNYV VTDHGSCVRACGADSYEMEEDGVRK CKKCEGPCRKVCNGIGIGEFKDSLS INATNIKHFKNCTSISGDLHILPVA FRGDSFTHTPPLDPQELDILKTVKE ITGFLLIQAWPENRTDLHAFENLEI IRGRTKQHGQFSLAVVSLNITSLGL RSLKEISDGDVIISGNKNLCYANTI NWKKLFGTSGQKTKIISNRGENSCK ATGQVCHALCSPEGCWGPEPRDCVS CRNVSRGRECVDKCNLLEGEPREFV ENSECIQCHPECLPQAMNITCTGRG PDNCIQCAHYIDGPHCVKTCPAGVM GENNTLVWKYADAGHVCHLCHPNCT YGCTGPGLEGCPTNGPKIPSIATGM VGALLLLLVVALGIGLFMRRRHIVR KRTLRRLLQERELVEPLTPSGEAPN QALLRILKETEFKKIKVLGSGAFGT VYKGLWIPEGEKVKIPVAIKELREA TSPKANKEILDEAYVMASVDNPHVC RLLGICLTSTVQLITQLMPFGCLLD YVREHKDNIGSQYLLNWCVQIAKGM NYLEDRRLVHRDLAARNVLVKTPQH VKITDFGLAKLLGAEEKEYHAEGGK VPIKWMALESILHRIYTHQSDVWSY GVTVWELMTFGSKPYDGIPASEISS ILEKGERLPQPPICTIDVYMIMVKC WMIDADSRPKFRELIIEFSKMARDP QRYLVIQGDERMHLPSPTDSNFYRA LMDEEDMDDVVDADEYLIPQQGFFS SPSTSRTPLLSSLSATSNNSTVACI DRNGLQSCPIKEDSFLQRYSSDPTG ALTEDSIDDTFLPVPEYINQSVPKR PAGSVQNPVYHNQPLNPAPSRDPHY QDPHSTAVGNPEYLNTVQPTCVNST FDSPAHWAQKGSHQISLDNPDYQQD FFPKEAKPNGIFKGSTAENAEYLRV APQSSEFIGA
• Purity: > 98%
• Applications: WB; ELISA; FACS; FC
• Stability: This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use.
• Storage: At -20 centigrade.
• Concentration: 1 mg/mL
• Storage Buffer: PBS (pH 7.4-7.5). Sterile-filtered colorless solution.
• Reconstitution: Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens (human) ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533

Reviews

09/05/2022

The powerful biological activity of EGFR combined with its high purity makes the experimental results highly reproducible, which is of great significance in the field of scientific research.

09/26/2019

Due to the short half-life and high clearance characteristics of EGFR, they can better adapt to the needs of clinical treatment and provide more treatment options for patients.

03/23/2019

The production process of EGFR uses advanced biotechnology, which makes the production process more efficient and environmentally friendly, and reduces the consumption of human and material resources.

Q&As

What biological processes of human embryonic development and tissue regeneration is EGFR associated with?

It is associated with biological processes in human embryonic development and tissue regeneration.

Why does EGFR-inhibiting drug therapy have adverse reactions?

EGFR inhibits adverse drug reactions such as rash, inflammation, and diarrhea during drug treatment.

What is the role of EGFR in tumor treatment?

The role of EGFR in tumor treatment is to act as a molecular marker and can be used as a prognostic indicator and monitor tumor progression; at the same time, EGFR mutations can also be targeted for targeted therapy.

What are the general methods used for EGFR gene mutation testing?

EGFR gene mutation detection generally uses PCR, fluorescence in situ hybridization, and direct sequencing.

What other drugs can EGFR inhibitors be combined with to increase efficacy?

The combination of EGFR inhibitors with chemotherapy drugs, radiotherapy, immunotherapy, etc. can increase the efficacy of treatment and reduce the occurrence of adverse reactions.

How do EGFR mutations affect the efficacy of targeted therapies?

EGFR mutations can have a significant impact on the efficacy of targeted therapies, and patients with mutations have higher response rates.