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Active Recombinant Human EGFR Protein (Leu 25 - Ser 645), His-tagged, low endotoxin

Cat.No. : EGFR-338H
Product Overview : Recombinant Human EGFR Protein, His-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). Predicted N-terminus: Leu 25. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Specification
  • Gene Information
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with a linear range of 0.8-6 ng/mL (QC tested).
Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Molecular Mass : 70.5kDa
Protein length : Leu 25 - Ser 645
Endotoxin : Less than 0.01 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name : EGFR epidermal growth factor receptor [ Homo sapiens ]
Official Symbol : EGFR
Synonyms : EGFR; epidermal growth factor receptor; ERBB1; ERBB; HER1; mENA; PIG61;
Gene ID : 1956
mRNA Refseq : NM_005228
Protein Refseq : NP_005219
MIM : 131550
UniProt ID : P00533

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What biological processes of human embryonic development and tissue regeneration is EGFR associated with? 10/07/2019

It is associated with biological processes in human embryonic development and tissue regeneration.

Why does EGFR-inhibiting drug therapy have adverse reactions? 09/25/2019

EGFR inhibits adverse drug reactions such as rash, inflammation, and diarrhea during drug treatment.

What is the role of EGFR in tumor treatment? 08/27/2019

The role of EGFR in tumor treatment is to act as a molecular marker and can be used as a prognostic indicator and monitor tumor progression; at the same time, EGFR mutations can also be targeted for targeted therapy.

What are the general methods used for EGFR gene mutation testing? 03/22/2019

EGFR gene mutation detection generally uses PCR, fluorescence in situ hybridization, and direct sequencing.

What other drugs can EGFR inhibitors be combined with to increase efficacy? 02/21/2019

The combination of EGFR inhibitors with chemotherapy drugs, radiotherapy, immunotherapy, etc. can increase the efficacy of treatment and reduce the occurrence of adverse reactions.

How do EGFR mutations affect the efficacy of targeted therapies? 02/04/2019

EGFR mutations can have a significant impact on the efficacy of targeted therapies, and patients with mutations have higher response rates.

Customer Reviews (3)

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Reviews
09/05/2022

    The powerful biological activity of EGFR combined with its high purity makes the experimental results highly reproducible, which is of great significance in the field of scientific research.

    09/26/2019

      Due to the short half-life and high clearance characteristics of EGFR, they can better adapt to the needs of clinical treatment and provide more treatment options for patients.

      03/23/2019

        The production process of EGFR uses advanced biotechnology, which makes the production process more efficient and environmentally friendly, and reduces the consumption of human and material resources.

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