Recombinant Human EGFR Protein (Leu 25 - Ser 645), His-tagged, low endotoxin
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|Product Overview :||Recombinant Human EGFR Protein, His-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). Predicted N-terminus: Leu 25. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity :||Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with a linear range of 0.8-6 ng/mL (QC tested).
Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) (Routinely tested).
|Molecular Mass :||70.5kDa|
|Protein length :||Leu 25 - Ser 645|
|Endotoxin :||Less than 0.01 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Notes :||Please avoid repeated freeze-thaw cycles.|
|Stability :||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name :||EGFR epidermal growth factor receptor [ Homo sapiens ]|
|Official Symbol :||EGFR|
|Synonyms :||EGFR; epidermal growth factor receptor; ERBB1; ERBB; HER1; mENA; PIG61;|
|Gene ID :||1956|
|mRNA Refseq :||NM_005228|
|Protein Refseq :||NP_005219|
|UniProt ID :||P00533|
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