Active Recombinant Human EGFR Protein (Leu 25 - Ser 645), His-tagged, low endotoxin
Cat.No. : | EGFR-338H |
Product Overview : | Recombinant Human EGFR Protein, His-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). Predicted N-terminus: Leu 25. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
- Specification
- Gene Information
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with a linear range of 0.8-6 ng/mL (QC tested). Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) (Routinely tested). |
Molecular Mass : | 70.5kDa |
Protein length : | Leu 25 - Ser 645 |
Endotoxin : | Less than 0.01 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | EGFR epidermal growth factor receptor [ Homo sapiens ] |
Official Symbol : | EGFR |
Synonyms : | EGFR; epidermal growth factor receptor; ERBB1; ERBB; HER1; mENA; PIG61; |
Gene ID : | 1956 |
mRNA Refseq : | NM_005228 |
Protein Refseq : | NP_005219 |
MIM : | 131550 |
UniProt ID : | P00533 |
Products Types
◆ Lysates | ||
EGFR-1462RCL | Recombinant Rat EGFR cell lysate | +Inquiry |
EGFR-663HCL | Recombinant Human EGFR cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionIt is associated with biological processes in human embryonic development and tissue regeneration.
EGFR inhibits adverse drug reactions such as rash, inflammation, and diarrhea during drug treatment.
The role of EGFR in tumor treatment is to act as a molecular marker and can be used as a prognostic indicator and monitor tumor progression; at the same time, EGFR mutations can also be targeted for targeted therapy.
EGFR gene mutation detection generally uses PCR, fluorescence in situ hybridization, and direct sequencing.
The combination of EGFR inhibitors with chemotherapy drugs, radiotherapy, immunotherapy, etc. can increase the efficacy of treatment and reduce the occurrence of adverse reactions.
EGFR mutations can have a significant impact on the efficacy of targeted therapies, and patients with mutations have higher response rates.
Customer Reviews (3)
Write a reviewThe powerful biological activity of EGFR combined with its high purity makes the experimental results highly reproducible, which is of great significance in the field of scientific research.
Due to the short half-life and high clearance characteristics of EGFR, they can better adapt to the needs of clinical treatment and provide more treatment options for patients.
The production process of EGFR uses advanced biotechnology, which makes the production process more efficient and environmentally friendly, and reduces the consumption of human and material resources.
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