Recombinant Human EGFRviii protein, His-Avi-tagged

• Cat.No.: EGFR-688H
• Product Overview: Human EGFR vIII Protein is expressed from mammalian with a 6-His and Avi tag at the N-terminal. It contains Leu 25 - Ser 645 (30-297: Val Cys Gln.. Cys Pro Arg → Gly).

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: It contains Leu 25 - Ser 645 (30-297: Val Cys Gln...Cys Pro Arg → Gly).
• Description: The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: The protein has a predicted MW of 41.6 kDa .As a result of glycosylation, the protein migrates as 65-80 kDa under reducing (R) condition (Bis-Tris PAGE).
• Endotoxin: Less than 0.05 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFRvIII,EGFR
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533