Recombinant Human EIF2S1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human EIF2S1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : EIF2S1-3542H
Product Overview : EIF2S1 MS Standard C13 and N15-labeled recombinant protein (NP_004085) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2), and the 52-kD EIF2-gamma subunit (EIF2S3). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 36.1 kDa
AA Sequence : MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY DAFKHAVSDP SILDSLDLNE DEREVLINNI NRRLTPQAVK IRADIEVACY GYEGIDAVKE ALRAGLNCST ENMPIKINLI APPRYVMTTT TLERTEGLSV LSQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQM ERLERENAEV DGDDDAEEME AKAEDTRTRP LEQKLISEED LAANDILDYK DDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : EIF2S1 eukaryotic translation initiation factor 2 subunit alpha [ Homo sapiens (human) ]
Official Symbol : EIF2S1
Synonyms : EIF2S1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2, eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD ); eukaryotic translation initiation factor 2 subunit 1; EIF 2alpha; EIF2A; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha; EIF2; EIF-2; EIF-2A; EIF-2alpha;
Gene ID : 1965
mRNA Refseq : NM_004094
Protein Refseq : NP_004085
MIM : 603907
UniProt ID : P05198

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