Active Recombinant Human FGF21 protein, His-tagged
Cat.No. : | FGF21-3279H |
Product Overview : | Recombinant Human FGF21 protein(His29~Ser209), fused with N-terminal His Tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
Bio-activity : | Fibroblast growth factor 21(FGF21) is a protein that inmammals is encoded by the FGF21 gene. The proteinencoded by this gene is a member of the fibroblastgrowth factor (FGF) family and specifically a memberof the endocrine subfamily which includes FGF23 andFGF15/19. FGF21 is the primary endogenous agonist ofthe FGF21 receptor, which is composed of the co-receptors FGF receptor 1 and β-Klotho. Besides, Klotho Beta (KLb) has been identified as an interactor of FGF21, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF21 and recombinant human KLb. Briefly, FGF21 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl then transferred to KLb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-KLb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of KLb and FGF21 was shown in Figure 1, and this effect was in a dose dependent manner. |
Molecular Mass : | 27kDa |
Protein Length : | His29~Ser209 |
Purity : | > 90% |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name : | FGF21 fibroblast growth factor 21 [ Homo sapiens ] |
Official Symbol : | FGF21 |
Synonyms : | FGF21; fibroblast growth factor 21; FGF-21; |
Gene ID : | 26291 |
mRNA Refseq : | NM_019113 |
Protein Refseq : | NP_061986 |
MIM : | 609436 |
UniProt ID : | Q9NSA1 |
Products Types
◆ Recombinant Protein | ||
Fgf21-414F | Active Recombinant Mouse Fgf21 Protein (182 aa) | +Inquiry |
FGF21-287F | Active Recombinant Human FGF21 Protein, His-tagged | +Inquiry |
FGF21-120F | Active Recombinant Human FGF21 Protein (182 aa) | +Inquiry |
Fgf21-1491R | Recombinant Rat Fgf21 Protein, His-tagged | +Inquiry |
FGF21-117H | Active Recombinant Human FGF21 Protein (His29-Ser209), C-His tagged, Animal-free, Carrier-free | +Inquiry |
◆ Lysates | ||
FGF21-1890MCL | Recombinant Mouse FGF21 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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