Active Recombinant Human FLT3LG protein, hFc-tagged, Low Endotoxin

• Cat.No.: FLT3LG-764H
• Product Overview: Recombinant Human FLT3LG protein(P49771-1)(Thr27-Pro185), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 27-185 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose and 0.01% tween is added as protectant before lyophilization.
• Bio-activity: Immobilized Human FLT3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 35.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 44.8 kDa. Due to glycosylation, the protein migrates to 90-118 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]
• Official Symbol: FLT3LG
• Synonyms: FLT3LG; fms-related tyrosine kinase 3 ligand; flt3 ligand; FL; FLT3L
• Gene ID: 2323
• mRNA Refseq: NM_001204502
• Protein Refseq: NP_001191431
• MIM: 600007
• UniProt ID: P49771