Recombinant Human GAPDH Protein, Myc/DDK-tagged, C13 and N15-labeled
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| Cat.No. : | GAPDH-4150H |
| Product Overview : | GAPDH MS Standard C13 and N15-labeled recombinant protein (NP_002037) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
| Description : | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
| Source : | HEK293 |
| Species : | Human |
| Tag : | Myc/DDK |
| Molecular Mass : | 36.1 kDa |
| AA Sequence : | MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKETRTRP LEQKLISEED LAANDILDYK DDDDKV |
| Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : | 50 μg/mL as determined by BCA |
| Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
| SDS-PAGE : |
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| Gene Name : | GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens (human) ] |
| Official Symbol : | GAPDH |
| Synonyms : | GAPDH; glyceraldehyde-3-phosphate dehydrogenase; GAPD; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; G3PD; MGC88685; |
| Gene ID : | 2597 |
| mRNA Refseq : | NM_002046 |
| Protein Refseq : | NP_002037 |
| MIM : | 138400 |
| UniProt ID : | P04406 |
◆ Recombinant Protein
| GAPDH-517C | Recombinant Chicken GAPDH Protein (2-333 aa), His-tagged | +Inquiry |
| GAPDH-9157H | Recombinant Human GAPDH, His-tagged | +Inquiry |
| Gapdh-3150M | Recombinant Mouse Gapdh Protein, Myc/DDK-tagged | +Inquiry |
| GAPDH-2519H | Recombinant Human GAPDH protein, His-tagged | +Inquiry |
| GAPDH-6540Z | Recombinant Zebrafish GAPDH | +Inquiry |
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◆ Native Protein
| GAPDH-126R | Native Rabbit GAPDH | +Inquiry |
| GAPDH-62H | Native Human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) | +Inquiry |
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◆ Lysates
| GAPDH-6024HCL | Recombinant Human GAPDH 293 Cell Lysate | +Inquiry |
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◆ Assay kits
| Kit-2216 | GAPDH Activity Assay Kit | +Inquiry |
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