Recombinant Human GCDH Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human GCDH Protein, Myc/DDK-tagged, C13 and N15-labeled

GCDH Related Products

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Cat.No. : GCDH-6691H
Product Overview : GCDH MS Standard C13 and N15-labeled recombinant protein (NP_000150) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Mutations in this gene result in the metabolic disorder glutaric aciduria type 1, which is also known as glutaric acidemia type I. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 12.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 48.1 kDa
AA Sequence : MALRGVSVRL LSRGPGLHVL RTWVSSAAQT EKGGRTQSQL AKSSRPEFDW QDPLVLEEQL TTDEILIRDT FRTYCQERLM PRILLANRNE VFHREIISEM GELGVLGPTI KGYGCAGVSS VAYGLLAREL ERVDSGYRSA MSVQSSLVMH PIYAYGSEEQ RQKYLPQLAK GELLGCFGLT EPNSGSDPSS METRAHYNSS NKSYTLNGTK TWITNSPMAD LFVVWARCED GCIRGFLLEK GMRGLSAPRI QGKFSLRASA TGMIIMDGVE VPEENVLPGA SSLGGPFGCL NNARYGIAWG VLGASEFCLH TARQYALDRM QFGVPLARNQ LIQKKLADML TEITLGLHAC LQLGRLKDQD KAAPEMVSLL KRNNCGKALD IARQARDMLG GNGISDEYHV IRHAMNLEAV NTYEGTHDIH ALILGRAITG IQAFTASKTR TRPLEQKLIS EEDLAANDIL DYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : GCDH glutaryl-CoA dehydrogenase [ Homo sapiens (human) ]
Official Symbol : GCDH
Synonyms : GCDH; glutaryl-CoA dehydrogenase; glutaryl Coenzyme A dehydrogenase; glutaryl-CoA dehydrogenase, mitochondrial; ACAD5; glutaryl-Coenzyme A dehydrogenase; GCD;
Gene ID : 2639
mRNA Refseq : NM_000159
Protein Refseq : NP_000150
MIM : 608801
UniProt ID : Q92947

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