Recombinant Human HDAC3 and NCOR2 Protein, His/GST-tagged

• Cat.No.: HDAC3-162H
• Product Overview: Complex of full-length recombinant C-terminal His-tagged HDAC3 and recombinant N-terminal GST-tagged NCOR2 amino acids 395-489, co-expressed in baculovirus expression system.

Specification

• Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
• Source: Insect cell
• Species: Human
• Tag: His/GST
• Molecular Mass: 49.7 kDa (HDAC3); 37.6 kDa (NCOR2)
• Purity: ≥90%
• Unit Definition: One unit is the amount of enzyme required to release 1 pmol of acetate per minute at 37 centigrade in 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/mL BSA, and 20 μM fluorogenic HDAC substrate 3.
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM sodium chloride, 2.2 mM potassium chloride, 250 mM imidazole, and 20% glycerol

Gene Information

• Gene Name: HDAC3 histone deacetylase 3 [ Homo sapiens (human) ]
• Official Symbol: HDAC3
• Synonyms: HDAC3; histone deacetylase 3; HD3; RPD3; KDAC3; RPD3-2; histone deacetylase 3; SMAP45; EC 3.5.1.98
• Gene ID: 8841
• mRNA Refseq: NM_003883
• Protein Refseq: NP_003874
• MIM: 605166
• UniProt ID: O15379