Recombinant Human HDAC5 Protein


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Recombinant Human HDAC5 Protein

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Cat.No. : HDAC5-312H
Product Overview : Recombinant Human HDAC5 Protein is produced by HEK293 cells expression system.
Description : Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
Source : HEK293 cells
Species : Human
Form : Liquid, In 30 mM HEPES, 140 mM NaCl, 10 mM KCl, 3% glycerol, 0.25 mM TCEP, pH 7.6.
Bio-activity : Deacetylation activity was determined using the Boc-K(TFA)-AMC substrate. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and the fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by nonlinear regression analysis.
Molecular Mass : 45 kDa
Protein length : amino acids 679-1095
Purity : ≥95% by SDS-PAGE
Notes : For Research Use Only! Not For Use in Humans.
Storage : Store at -80 centigrade.
Handling : Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles.
Gene Name : HDAC5 histone deacetylase 5 [ Homo sapiens ]
Official Symbol : HDAC5
Synonyms : HDAC5; histone deacetylase 5; FLJ90614; KIAA0600; NY CO 9; antigen NY-CO-9; HD5; NY-CO-9;
Gene ID : 10014
mRNA Refseq : NM_001015053
Protein Refseq : NP_001015053
MIM : 605315
UniProt ID : Q9UQL6

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