| Cat.No.: | IgG1Fc-12H |
| Product Overview: | Biotinylated Human IgG1 Fc is expressed from HEK293 cells. It contains AA Glu 99 - Lys 330 (Accession # P01857-1 ). This protein carries an Avi tag at the C-terminus. |
| Source: | HEK293 |
| Species: | Human |
| Tag : | Avi |
| Predicted N Terminal: | Glu 99 |
| Form: | Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose. |
| Bio-activity: | Immobilized Recombinant Protein G at 2 ug/mL (100 uL/well) can bind Biotinylated Human IgG1 Fc, Avi Tag with a linear range of 13-512 ug/mL. |
| Molecular Mass: | The protein has a calculated MW of 28.4 kDa. The protein migrates as 36-40 kDa under reducing condition (SDS-PAGE) due to glycosylation. |
| Endotoxin: | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity: | >95% as determined by SDS-PAGE. |
| Characteristic: | Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
| Stability: | Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage: | For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| Official Symbol: | IgG1Fc |
| Synonyms: | IgG Fc |
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