Recombinant Human Interleukin-11

• Cat.No.: IL11-19H
• Product Overview: The protein is produced inP. Pichiaby recombinant DNA technology. The protein has a molecular mass of approximately 19,000 Daltons and is non-glycosylated. The protein is 177 amino acids in length and differs from the 178 amino acid length of native IL-11 only in lacking the amino-terminal praline residue.

Specification

• Cat. No.: IL11-19H
• Description: IL-11 is a thrombopoietic growth factor that directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. IL-11 is a member of a family of human growth factors which includes human growth hormone, granulocyte colony-stimulating factor and other growth factors.
• Purity: >95% as determined by SDS-PAGE and SEC-HPLC.
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
• Specific Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of murine 7TD1 was found to be less than 0.2ng/ml, corresponding to a Specific Activity of 8.0 x 10⁶IU/ mg.
• Endotoxin: Less than 2ng/mg (2IEU/mg) determined by LAL test.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-11 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Lyophilized rHuIL-11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: IL11
• Synonyms: AGIF; IL-11; Adipogenesis inhibitory factor, Oprelvekin, Interleukin-11; IL11; interleukin 11
• Gene ID: 3589
• mRNA Refseq: NM_000641
• Protein Refseq: NP_000632
• MIM: 147681
• UniProt ID: P20809
• Chromosome Location: 19q13.3-q13.4
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway
• Function: cytokine activity, interleukin-11 receptor binding activity