Recombinant Human IL11 protein(Pro22-Leu199)

• Cat.No.: IL11-464H
• Product Overview: Recombinant Human IL11 (AAH12506.1) (Pro22-Leu199) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: Non
• Protein length: Pro22-Leu199
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 1-8 ng/mL.
• Molecular Mass: The recombinant human IL11 consists of 178 amino acids and predicts a molecular mass of 19.1 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL11 interleukin 11 [ Homo sapiens ]
• Official Symbol: IL11
• Synonyms: IL11; interleukin 11; interleukin-11; adipogenesis inhibitory factor; AGIF; IL 11; oprelvekin; IL-11
• Gene ID: 3589
• mRNA Refseq: NM_000641
• Protein Refseq: NP_000632
• MIM: 147681
• UniProt ID: P20809

Reviews

10/28/2019

Potent in bone regeneration models.

11/26/2017

Suitable for cell culture.

01/10/2017

High yield in expression.

Q&As

How are in vivo models, including transgenic mice and knockdown strategies, utilized to study IL11's effects on tissue regeneration and homeostasis?

Transgenic mice and knockdown models explore IL11's impact on tissue regeneration.

Could you explain the experimental approaches, such as site-directed mutagenesis, employed to identify key residues in IL11's interaction with IL11RA?

Site-directed mutagenesis identifies key residues in IL11's interaction with IL11RA.

What techniques like chromatin immunoprecipitation and DNA methylation analysis contribute to deciphering IL11's regulatory mechanisms?

Chromatin immunoprecipitation and DNA methylation analysis decipher IL11's regulatory mechanisms.

Can you elaborate on the role of proteomics in identifying IL11-interacting proteins and potential cross-talk with other signaling pathways?

Proteomics identifies IL11-interacting proteins and potential cross-talk partners.

How do cell-based assays and luciferase reporter assays help investigate IL11's downstream signaling pathways through JAK-STAT activation?

Cell-based and luciferase reporter assays elucidate IL11's JAK-STAT signaling activation.

What role does RNA sequencing play in understanding IL11-induced gene expression changes in various cell types?

RNA sequencing uncovers IL11-induced gene expression changes across different cell types.

What structural insights have X-ray crystallography and nuclear magnetic resonance spectroscopy provided about the IL11-IL11RA complex?

X-ray crystallography and NMR spectroscopy reveal structural details of the IL11-IL11RA complex.

How do advanced imaging techniques like confocal microscopy and super-resolution microscopy visualize IL11 localization and distribution in tissues?

Confocal and super-resolution microscopy visualize IL11 localization in tissues.

How are techniques like surface plasmon resonance used to study IL11's binding kinetics to its receptor IL11RA?

Techniques like surface plasmon resonance measure IL11-IL11RA binding affinity and kinetics.

What insights have CRISPR screening approaches provided regarding genes and pathways influenced by IL11 signaling?

CRISPR screening unveils genes and pathways influenced by IL11 signaling.