| Product overview : |
Recombinant Human IL12A (P29459) aa. (IL27B subunit (Arg21~Lys229) and the mature form of human IL12A (Arg23~Ser219) linked by a polypeptide linker (GGGGS)3) fused with His tag was produced in E. coli cells. |
| Description : |
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Freeze-dried powder |
| Molecular Mass : |
Predicted Molecular Mass: 51.7kDa; Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions. |
| Protein length : |
The IL35 complex composed of IL27B subunit (Arg21~Lys229) and the mature form of human IL12A (Arg23~Ser219) linked by a polypeptide linker (GGGGS)3 |
| Purity : |
>98% |
| Characteristic : |
Isoelectric point is 8.7. |
| Applications : |
Cell culture; Activity Assays; In vivo assays. |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Storage buffer : |
20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |
