Recombinant Human IL15RA protein, His-tagged

• Cat.No.: IL15RA-14151H
• Product Overview: Recombinant Human IL15RA protein(1-231 aa), fused with His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 1-231 aa
• AA Sequence: MSVEHADIWVKSYSLYSRERYICNS GFKRKAGTSSLTECVLNKATNVAHW TTPSLKCIRDPALVHQRPAPPSTVT TAGVTPQPESLSPSGKEPAASSPSS NNTAATTAAIVPGSQLMPSKSPSTG TTEISSHESSHGTPSQTTAKTWELT ASASHQPPGVYPQGHSDTTVAISTS TVLLCGLSAVSLLACYLKSRQTPPL ASVEMEAMEALPVTWGTSSRDEDLE NCSHHL
• Purity: 80%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: IL15RA interleukin 15 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL15RA
• Synonyms: IL15RA; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; CD215; IL 15RA; MGC104179
• Gene ID: 3601
• mRNA Refseq: NM_001243539
• Protein Refseq: NP_001230468
• MIM: 601070
• UniProt ID: Q13261

Reviews

10/29/2020

Effective in T-cell assays.

03/10/2018

High purity levels.

05/28/2017

Consistent receptor binding.

Q&As

How does immunoprecipitation validate IL15RA's association with IL2RB?

Immunoprecipitation validates IL15RA's binding to IL2RB in cells.

What are the applications of CRISPR/Cas9 in studying IL15RA knockout models?

CRISPR/Cas9 technology aids in generating IL15RA knockout models.

Could you explain the approaches to investigate IL15RA's role in autoimmune diseases?

Approaches unravel IL15RA's implication in autoimmune disease mechanisms.

What experimental strategies differentiate IL15RA's role in innate and adaptive immunity?

Methods discern IL15RA's distinct roles in innate and adaptive immunity.

What techniques characterize IL15RA isoform expression in tissues?

RT-PCR and Western blotting assess IL15RA isoform expression patterns.

How is IL15RA's binding affinity to IL15 measured in vitro?

Binding affinity of IL15RA to IL15 quantified through surface plasmon resonance.

What methods explore IL15RA's involvement in immune cell proliferation?

Proliferation assays investigate IL15RA's contribution to immune cell growth.

How is IL15RA's interaction with JAK/STAT pathways analyzed?

IL15RA's interaction with JAK/STAT pathways probed using immunoblotting.

How do siRNA knockdown experiments reveal IL15RA's role in signaling?

siRNA knockdown studies unveil IL15RA's impact on downstream signaling.

Can you detail the use of FRET assays to study IL15RA dimerization?

FRET assays employed to elucidate IL15RA dimerization kinetics.