Recombinant Human IL18 protein

• Cat.No.: IL18-186H
• Product Overview: Recombinant Human IL18 protein(Q14116)(Tyr37-Asp193) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: Tyr37-Asp193
• Tag: Non
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
• Official Symbol: IL18
• Synonyms: IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IGIF; IL 1g; IL 18; IL1F4; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; IL-18; IL-1g; MGC12320
• Gene ID: 3606
• mRNA Refseq: NM_001243211
• Protein Refseq: NP_001230140
• MIM: 600953
• UniProt ID: Q14116

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.