Recombinant Human IL18BP protein, Fc-tagged

Cat.No. : IL18BP-162H
Product Overview : Recombinant Human IL18BP (Thr31-Gly194) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
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Description : Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Thr31
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 44.3 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Thr31-Gly194
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
SDS-PAGE: Human IL-18BP, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Gene Name : IL18BP
Official Symbol : IL18BP
Synonyms : IL18BP; interleukin 18 binding protein; interleukin-18-binding protein; IL18BPa; MC51L 53L 54L homolog gene product; IL-18BP; tadekinig-alfa; MC51L-53L-54L homolog gene product
Gene ID : 10068
mRNA Refseq : NM_001039659
Protein Refseq : NP_001034748
MIM : 604113
UniProt ID : O95998

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can you elaborate on the experimental strategies used to assess the genetic polymorphisms and variations in IL18BP that might influence its function and disease susceptibility? 11/23/2021

Genetic association studies, sequencing analyses, and functional investigations explore how genetic variations in IL18BP might influence disease susceptibility and outcomes.

How are animal models and in vivo imaging techniques used to understand the role of IL18BP in modulating IL18-mediated immune responses in whole organisms? 10/17/2021

Knockout mice, transgenic models, and imaging techniques provide insights into IL18BP's impact on IL18-related immune responses in vivo.

What techniques are used to investigate the regulation of IL18BP expression by cytokines, growth factors, and other signaling molecules in different cell types? 09/06/2021

Promoter studies, siRNA knockdown, and cytokine stimulation assays investigate IL18BP regulation at the transcriptional and post-transcriptional levels.

How are cell-based assays and functional studies employed to dissect the mechanisms through which IL18BP modulates IL18-induced immune responses? 04/29/2021

Luciferase reporter assays, ELISAs, and functional cell assays probe how IL18BP modulates IL18-induced cytokine production and immune cell activation.

What experimental approaches are utilized to explore the potential allosteric effects of IL18BP on IL18 receptor complex formation and downstream signaling? 04/10/2021

Biophysical techniques, site-directed mutagenesis, and receptor binding assays reveal potential allosteric effects on IL18 signaling.

How do researchers experimentally characterize the binding affinity and kinetics between IL18BP and IL18, considering their critical role in regulating IL18-mediated inflammation? 09/07/2019

Surface plasmon resonance, isothermal titration calorimetry, and kinetic binding assays quantify IL18BP-IL18 interactions.

Can you explain the strategies used to assess the expression levels and tissue distribution of IL18BP in various physiological and pathological contexts? 03/08/2019

RT-qPCR, immunohistochemistry, and tissue microarray analyses determine the expression patterns of IL18BP in different tissues and diseases.

Could you elaborate on the methods used to investigate the structural determinants of IL18BP's interaction with IL18 and its impact on receptor binding? 12/12/2017

X-ray crystallography, NMR spectroscopy, and mutagenesis studies elucidate the structural basis of IL18BP-IL18 recognition.

How do researchers experimentally study the potential cross-talk between IL18BP and other cytokine regulatory systems, such as IL1Ra and soluble IL6 receptor? 09/24/2017

Cross-competition assays, co-stimulation studies, and receptor activation assays unveil potential interplay between IL18BP and other cytokine regulators.

What assays and methodologies are employed to evaluate the therapeutic potential of enhancing or inhibiting IL18BP activity in inflammatory diseases? 08/26/2017

Therapeutic antibody trials, in vitro functional screens, and disease model assessments evaluate IL18BP modulation as a potential anti-inflammatory strategy.

Customer Reviews (3)

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Reviews
06/03/2022

    Consistent binding affinity.

    03/01/2020

      High purity.

      05/04/2018

        Effective in neutralization assays.

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