|Product Overview:||Recombinant Human IL19(Leu25-Ala177) was expressed in NS0.|
|Description:||Interleukin 19 (IL-19) is a member of the IL-10 family of class II alpha -helical cytokines that contains two groups, a viral homolog and cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R beta as a signal transducing receptor (IL-10, IL-22, and IL-26), and one which uses IL-20 R beta as a signal transducing receptor (IL-19, IL-20, and IL-24). The 153 amino acid (aa) mature human IL-19 is secreted as a glycosylated 35-45 kDa monomer. It shares approximately 70% aa sequence identity with mouse and rat IL-19. Human IL-19 may utilize an alternate start site that adds 38 aa to the N-terminus. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 is expressed on activated keratinocytes, monocytes, and B cells. It binds a receptor complex consisting of IL-20 R alpha and IL-20 R beta. This receptor complex is also shared by IL-20 and IL-24. It has been reported that IL-19 both will and will not induce IL-6 and TNF-alpha production by monocytes. It does, however, drive T helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself.|
|Predicted N Terminal:||Leu25|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.|
|Bio-activity:||Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is typically 0.1-0.6 ng/mL.|
|Molecular Mass:||Predicted Molecular Mass: 18 kDa
SDS-PAGE: 20-30 kDa, reducing conditions
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 500 μg/mL in PBS.|
|Gene Name:||IL19 interleukin 19 [ Homo sapiens ]|
|Synonyms:||IL19; interleukin 19; interleukin-19; IL 10C; IL 19; MDA1; melanoma differentiation associated protein like protein; NG.1; ZMDA1; melanoma differentiation associated protein-like protein; melanoma differentiation-associated protein-like protein; IL-10C;|
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