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Recombinant Human IL1RAPL2 cell lysate

Cat.No. : IL1RAPL2-2913HCL
Product Overview : Human IL1R9 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Tag : Non
Gene Name : IL1RAPL2 interleukin 1 receptor accessory protein-like 2 [ Homo sapiens ]
Official Symbol : IL1RAPL2
Synonyms : IL1RAPL2; interleukin 1 receptor accessory protein-like 2; X-linked interleukin-1 receptor accessory protein-like 2; IL 1R9; IL1R9; IL1RAPL 2; TIGIRR 1; IL-1R-9; IL-1RAPL-2; IL-1-RAPL-2; interleukin 1 receptor 9; interleukin-1 receptor 9; IL1RAPL-2-related protein; IL-1 receptor accessory protein-like 2; three immunoglobulin domain-containing IL-1 receptor-related 1; IL-1R9; TIGIRR-1; IL1RAPL-2;
Gene ID : 26280
mRNA Refseq : NM_017416
Protein Refseq : NP_059112
MIM : 300277
UniProt ID : Q9NP60
Chromosome Location : Xq22
Function : interleukin-1 receptor activity; interleukin-1, Type II, blocking receptor activity; receptor activity; transmembrane signaling receptor activity;

Not For Human Consumption!

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Customer Reviews (3)

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07/02/2022

    Suitable for co-immunoprecipitation.

    11/09/2020

      High specificity.

      11/23/2016

        Good for flow cytometry.

        Q&As (10)

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        How does IL1RAPL2 affect T-cell activation? 08/23/2021

        IL1RAPL2 enhances T-cell activation by modulating calcium signaling.

        What role does IL1RAPL2 play in immune responses? 07/29/2020

        IL1RAPL2 modulates dendritic cell maturation in immune responses.

        Is IL1RAPL2 involved in autoimmune diseases? 06/04/2020

        IL1RAPL2 is implicated in multiple sclerosis.

        What is the subcellular localization of IL1RAPL2? 12/31/2019

        IL1RAPL2 is localized in the cytoplasm and cell membrane.

        Does IL1RAPL2 interact with any cytokines? 03/01/2019

        IL1RAPL2 does not directly interact with cytokines but influences their signaling.

        Are there post-translational modifications of IL1RAPL2? 07/24/2018

        IL1RAPL2 undergoes phosphorylation and ubiquitination.

        Is IL1RAPL2 expression altered in cancer? 06/28/2018

        IL1RAPL2 expression is upregulated in certain types of cancer.

        How does IL1RAPL2 influence cell migration? 06/17/2018

        IL1RAPL2 promotes cell migration through actin remodeling.

        How is IL1RAPL2 expression regulated in inflammatory conditions? 06/21/2016

        IL1RAPL2 expression is upregulated by pro-inflammatory cytokines.

        Does IL1RAPL2 have any enzymatic activity? 06/11/2016

        IL1RAPL2 does not have enzymatic activity.

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