Active Recombinant Human IL21 protein

• Cat.No.: IL21-371H
• Product Overview: Active Recombinant Human IL21 protein(Q9HBE4-1) (Gln 23-Ser 155) was expressed and purified in E. coli, with an initial Met.

Specification

• Species: Human
• Source: E. coli
• Tag: Non
• Protein Length: 23-155 aa
• Form: Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5.
• Bio-activity: 1. Measured by its ability to bind human IL-21R in a functional ELISA. 2. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.4-2 ng/ml.
• Molecular Mass: The recombinant human IL-21 consisting of 134 amino acids and has a calculated molecular mass of 15.6 kDa. It migrates as an approximately 17 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL21 interleukin 21 [ Homo sapiens ]
• Official Symbol: IL21
• Synonyms: IL21; interleukin 21; interleukin-21; IL 21; Za11; interleukin-21 isoform; IL-21
• Gene ID: 59067
• mRNA Refseq: NM_001207006
• Protein Refseq: NP_001193935
• MIM: 605384
• UniProt ID: Q9HBE4