Recombinant Human IL21 protein, His-tagged

• Cat.No.: IL21-15H
• Product Overview: Recombinant Human IL21 protein, fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human IL21R at 2 μg/mL (100 μL/well) can bind Human IL21 with a linear range of 0.078-0.973 μg/mL. 2.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 1.52-6.06 ng/mL, corresponding to a specific activity of 1.65×10^5~6.58×10^5 units/mg.
• Molecular Mass: 16.12 kDa
• Endotoxin: < 1.0 EU/μg of the protein by LAL method.
• Purity: > 95% by SDS-PAGE.
• Storage: Store at -20°C. Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: IL21 interleukin 21 [ Homo sapiens ]
• Official Symbol: IL21
• Synonyms: IL21; interleukin 21; interleukin-21; IL 21; Za11; interleukin-21 isoform; IL-21
• Gene ID: 59067
• mRNA Refseq: NM_001207006
• Protein Refseq: NP_001193935
• MIM: 605384
• UniProt ID: Q9HBE4