Recombinant Human IL26 293 Cell Lysate
- Gene Information
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|Description :||Antigen standard for interleukin 26 (IL26) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.|
|Source :||HEK 293 cells|
|Components :||This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).|
|Size :||0.1 mg|
|Storage Instruction :||Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.|
|Applications :||ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.|
|Gene Name :||IL26 interleukin 26 [ Homo sapiens ]|
|Official Symbol :||IL26|
|Synonyms :||IL26; interleukin 26; interleukin-26; AK155; IL 26; IL-26;|
|Gene ID :||55801|
|mRNA Refseq :||NM_018402|
|Protein Refseq :||NP_060872|
|UniProt ID :||Q9NPH9|
|Chromosome Location :||12q15|
|Pathway :||Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;|
|Function :||cytokine activity;|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (6)Ask a question
The level of Il26 in vivo can be detected by enzyme-linked immunosorbent assay and flow cytometry.
Il26 can mediate the inflammatory response, regulating it by attracting immune cells and enhancing inflammation.
Il26 can be used for laboratory animal studies.
It can mediate cell growth and differentiation through JAK-STAT signal transduction pathway.
The production process of Il26 can be optimized by optimizing the transformation system, improving the culture conditions and optimizing the purification process.
Drugs targeting Il26 can be designed through computer simulation, drug screening and molecular modification.
Customer Reviews (3)Write a review
Animal models are used to evaluate the biological function of IL-26 protein, and its effects and side effects are evaluated by observing its effects on immune response, inflammation, disease, etc., in mice or rats. -
Techniques such as ELISA or flow cytometry can be used to detect IL-26 concentrations in body fluids or cells. -
The molecular weight, composition and stability of IL-26 protein can be determined by western blotting and mass spectrometry. -
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