Active Recombinant Human IL28B protein, His-tagged

• Cat.No.: IL28B-379H
• Product Overview: Active Recombinant Human IL28B protein(NP_742151.2) (Met 1-Val 196) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-196 aa
• Form: Lyophilized from sterile PBS, 500mM NaCl, pH 7.4.
• Bio-activity: 1. Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is typically 2-6 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized human IL28B at 20 μg/ml (100 μl/well) can bind human IL10RB with a linear ranger of 3.125-25 μg/ml.
• Molecular Mass: The recombinant human IL28B consists of 186 amino acids and predictes a molecular mass of 21 kDa. In SDS-PAGE under reducing conditions, rhIL28B migrates as an approximately 23 kDa band.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL28B interleukin 28B (interferon, lambda 3) [ Homo sapiens ]
• Official Symbol: IL28B
• Synonyms: IL28B; interleukin 28B (interferon, lambda 3); interleukin 28B; interleukin-28B; IFNL3; IL 28B; IL28C; IL-28C; IFN-lambda-3; IFN-lambda-4; interleukin 28C; interleukin-28C; cytokine Zcyto22; interferon lambda-3; interferon lambda-4; interferon, lambda 3; interferon, lambda 4; IL-28B
• Gene ID: 282617
• mRNA Refseq: NM_172139
• Protein Refseq: NP_742151
• MIM: 607402
• UniProt ID: Q8IZI9